Profiling of secreted proteins from human ovarian cancer cell lines by surface-enhanced laser desorption ionization time-of-flight mass spectrometry

Da Rue Prieto; Thomas P. Conrads; Dominic A. Scudiero; Timothy D. Veenstra; Haleem J. Issaq

doi:10.1081/JLC-120023249

Profiling of secreted proteins from human ovarian cancer cell lines by surface-enhanced laser desorption ionization time-of-flight mass spectrometry

Da Rue Prieto, Thomas P. Conrads, Dominic A. Scudiero, Timothy D. Veenstra, Haleem J. Issaq^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Surface-enhanced laser desorption ionization (SELDI) time-of-flight mass spectrometry (TOF MS) was used to profile six human ovarian cancer cell lines. Non-confluent cell cultures were exchanged into serum free medium and allowed to grow for either 24 or 48 hours, at which time the medium was collected and analyzed using a Ciphergen SELDI-TOF MS and a QSTAR Pulsar QqTOF mass spectrometer fitted with a SELDI source. The spectra showed the presence of several low molecular weight species, as well as differences and similarities in the proteins detected in the media of the six ovarian tumor cell lines. The same species were detected at 24 and 48 hours and reproducible changes in their relative abundances were observed.

Original language	English
Pages (from-to)	2315-2328
Number of pages	14
Journal	Journal of Liquid Chromatography and Related Technologies
Volume	26
Issue number	14
DOIs	https://doi.org/10.1081/JLC-120023249
State	Published - 2003
Externally published	Yes

Keywords

Biomarker detection
Cancer
Protein profile

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10.1081/JLC-120023249

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title = "Profiling of secreted proteins from human ovarian cancer cell lines by surface-enhanced laser desorption ionization time-of-flight mass spectrometry",

abstract = "Surface-enhanced laser desorption ionization (SELDI) time-of-flight mass spectrometry (TOF MS) was used to profile six human ovarian cancer cell lines. Non-confluent cell cultures were exchanged into serum free medium and allowed to grow for either 24 or 48 hours, at which time the medium was collected and analyzed using a Ciphergen SELDI-TOF MS and a QSTAR Pulsar QqTOF mass spectrometer fitted with a SELDI source. The spectra showed the presence of several low molecular weight species, as well as differences and similarities in the proteins detected in the media of the six ovarian tumor cell lines. The same species were detected at 24 and 48 hours and reproducible changes in their relative abundances were observed.",

keywords = "Biomarker detection, Cancer, Protein profile",

author = "Prieto, {Da Rue} and Conrads, {Thomas P.} and Scudiero, {Dominic A.} and Veenstra, {Timothy D.} and Issaq, {Haleem J.}",

note = "Funding Information: By acceptance of this article, the publisher or recipient acknowledges the right of the U.S. Government to retain a nonexclusive, royalty-free license and to any copyright covering the article. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organization imply endorsement by the U.S. Government. This project has been funded in whole, or in part, with Federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. NO1-CO-12400.",

year = "2003",

doi = "10.1081/JLC-120023249",

language = "English",

volume = "26",

pages = "2315--2328",

journal = "Journal of Liquid Chromatography and Related Technologies",

issn = "1082-6076",

number = "14",

}

Profiling of secreted proteins from human ovarian cancer cell lines by surface-enhanced laser desorption ionization time-of-flight mass spectrometry. / Prieto, Da Rue; Conrads, Thomas P.; Scudiero, Dominic A. et al.
In: Journal of Liquid Chromatography and Related Technologies, Vol. 26, No. 14, 2003, p. 2315-2328.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Profiling of secreted proteins from human ovarian cancer cell lines by surface-enhanced laser desorption ionization time-of-flight mass spectrometry

AU - Prieto, Da Rue

AU - Conrads, Thomas P.

AU - Scudiero, Dominic A.

AU - Veenstra, Timothy D.

AU - Issaq, Haleem J.

N1 - Funding Information: By acceptance of this article, the publisher or recipient acknowledges the right of the U.S. Government to retain a nonexclusive, royalty-free license and to any copyright covering the article. The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organization imply endorsement by the U.S. Government. This project has been funded in whole, or in part, with Federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. NO1-CO-12400.

PY - 2003

Y1 - 2003

N2 - Surface-enhanced laser desorption ionization (SELDI) time-of-flight mass spectrometry (TOF MS) was used to profile six human ovarian cancer cell lines. Non-confluent cell cultures were exchanged into serum free medium and allowed to grow for either 24 or 48 hours, at which time the medium was collected and analyzed using a Ciphergen SELDI-TOF MS and a QSTAR Pulsar QqTOF mass spectrometer fitted with a SELDI source. The spectra showed the presence of several low molecular weight species, as well as differences and similarities in the proteins detected in the media of the six ovarian tumor cell lines. The same species were detected at 24 and 48 hours and reproducible changes in their relative abundances were observed.

AB - Surface-enhanced laser desorption ionization (SELDI) time-of-flight mass spectrometry (TOF MS) was used to profile six human ovarian cancer cell lines. Non-confluent cell cultures were exchanged into serum free medium and allowed to grow for either 24 or 48 hours, at which time the medium was collected and analyzed using a Ciphergen SELDI-TOF MS and a QSTAR Pulsar QqTOF mass spectrometer fitted with a SELDI source. The spectra showed the presence of several low molecular weight species, as well as differences and similarities in the proteins detected in the media of the six ovarian tumor cell lines. The same species were detected at 24 and 48 hours and reproducible changes in their relative abundances were observed.

KW - Biomarker detection

KW - Cancer

KW - Protein profile

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JF - Journal of Liquid Chromatography and Related Technologies

IS - 14

ER -

Profiling of secreted proteins from human ovarian cancer cell lines by surface-enhanced laser desorption ionization time-of-flight mass spectrometry

Abstract

Keywords

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