Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1

Galina Petukhova; Patrick Sung; Hannah Klein

doi:10.1101/gad.826100

Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1

Galina Petukhova, Patrick Sung, Hannah Klein^*

^*Corresponding author for this work

Biochemistry

Research output: Contribution to journal › Article › peer-review

122 Scopus citations

Abstract

The first DNA joint formed in homologous recombination processes is a D-loop. Saccharomyces cerevisiae RDH54/TID1-encoded product, a Swi2/Snf2-like factor involved in recombination, is shown here to promote D-loop formation with Rad51 recombinase. Physical interaction between Rdh54 and Rad51 is functionally important because Rdh54 does not enhance the recombinase activity of the Escherichia coli RecA protein. Robust dsDNA-activated ATPase activity in Rdh54 generates unconstrained negative and positive supercoils in DNA. Efficient D-loop formation occurs with even topologically relaxed DNA, suggesting that via specific protein-protein interactions, the negative supercoils produced by Rdh54 are used by Rad51 for making DNA joints.

Original language	English
Pages (from-to)	2206-2215
Number of pages	10
Journal	Genes and Development
Volume	14
Issue number	17
DOIs	https://doi.org/10.1101/gad.826100
State	Published - 2000

Keywords

DNA double-strand break repair
DNA supercoiling
Homologous recombination
Meiosis

Access to Document

10.1101/gad.826100

Cite this

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title = "Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1",

abstract = "The first DNA joint formed in homologous recombination processes is a D-loop. Saccharomyces cerevisiae RDH54/TID1-encoded product, a Swi2/Snf2-like factor involved in recombination, is shown here to promote D-loop formation with Rad51 recombinase. Physical interaction between Rdh54 and Rad51 is functionally important because Rdh54 does not enhance the recombinase activity of the Escherichia coli RecA protein. Robust dsDNA-activated ATPase activity in Rdh54 generates unconstrained negative and positive supercoils in DNA. Efficient D-loop formation occurs with even topologically relaxed DNA, suggesting that via specific protein-protein interactions, the negative supercoils produced by Rdh54 are used by Rad51 for making DNA joints.",

keywords = "DNA double-strand break repair, DNA supercoiling, Homologous recombination, Meiosis",

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T1 - Promotion of Rad51-dependent D-loop formation by yeast recombination factor Rdh54/Tid1

AU - Petukhova, Galina

AU - Sung, Patrick

AU - Klein, Hannah

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N2 - The first DNA joint formed in homologous recombination processes is a D-loop. Saccharomyces cerevisiae RDH54/TID1-encoded product, a Swi2/Snf2-like factor involved in recombination, is shown here to promote D-loop formation with Rad51 recombinase. Physical interaction between Rdh54 and Rad51 is functionally important because Rdh54 does not enhance the recombinase activity of the Escherichia coli RecA protein. Robust dsDNA-activated ATPase activity in Rdh54 generates unconstrained negative and positive supercoils in DNA. Efficient D-loop formation occurs with even topologically relaxed DNA, suggesting that via specific protein-protein interactions, the negative supercoils produced by Rdh54 are used by Rad51 for making DNA joints.

AB - The first DNA joint formed in homologous recombination processes is a D-loop. Saccharomyces cerevisiae RDH54/TID1-encoded product, a Swi2/Snf2-like factor involved in recombination, is shown here to promote D-loop formation with Rad51 recombinase. Physical interaction between Rdh54 and Rad51 is functionally important because Rdh54 does not enhance the recombinase activity of the Escherichia coli RecA protein. Robust dsDNA-activated ATPase activity in Rdh54 generates unconstrained negative and positive supercoils in DNA. Efficient D-loop formation occurs with even topologically relaxed DNA, suggesting that via specific protein-protein interactions, the negative supercoils produced by Rdh54 are used by Rad51 for making DNA joints.

KW - DNA double-strand break repair

KW - DNA supercoiling

KW - Homologous recombination

KW - Meiosis

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