Properties of cold-adapted subtilisine-like proteinase of endemic yeast Leucosporidium antarcticum.

M. Turkiewicz*, M. Pazgier, H. Kalinowska, S. Bielecki

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

1 Scopus citations


An extracellular serine proteinase from psychrophilic marine Antarctic yeast L. antarcticum has been purified to homogeneity and characterized. The enzyme specificity, which resembles both chymotrypsin and subtilisin, as well as kinetic (topt of 25 degrees C, activity up to -20 degrees C, pHoptBzTyrOEt of 8.0-8.5), and thermodynamic properties conferring cold-adaptation of the proteinase, were determined The comparison of N-terminal sequence of 35 amino acid residues with known sequences of serine proteinases indicates that the enzyme can be preliminarly classified as a subtilisin-like proteinase, belonging to the proteinase K subfamily (clan SB, family S8, subfamily C).

Original languageEnglish
Pages (from-to)329-332
Number of pages4
JournalMededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)
Issue number3 a
StatePublished - 2001
Externally publishedYes


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