TY - JOUR
T1 - Properties of cold-adapted subtilisine-like proteinase of endemic yeast Leucosporidium antarcticum.
AU - Turkiewicz, M.
AU - Pazgier, M.
AU - Kalinowska, H.
AU - Bielecki, S.
PY - 2001
Y1 - 2001
N2 - An extracellular serine proteinase from psychrophilic marine Antarctic yeast L. antarcticum has been purified to homogeneity and characterized. The enzyme specificity, which resembles both chymotrypsin and subtilisin, as well as kinetic (topt of 25 degrees C, activity up to -20 degrees C, pHoptBzTyrOEt of 8.0-8.5), and thermodynamic properties conferring cold-adaptation of the proteinase, were determined The comparison of N-terminal sequence of 35 amino acid residues with known sequences of serine proteinases indicates that the enzyme can be preliminarly classified as a subtilisin-like proteinase, belonging to the proteinase K subfamily (clan SB, family S8, subfamily C).
AB - An extracellular serine proteinase from psychrophilic marine Antarctic yeast L. antarcticum has been purified to homogeneity and characterized. The enzyme specificity, which resembles both chymotrypsin and subtilisin, as well as kinetic (topt of 25 degrees C, activity up to -20 degrees C, pHoptBzTyrOEt of 8.0-8.5), and thermodynamic properties conferring cold-adaptation of the proteinase, were determined The comparison of N-terminal sequence of 35 amino acid residues with known sequences of serine proteinases indicates that the enzyme can be preliminarly classified as a subtilisin-like proteinase, belonging to the proteinase K subfamily (clan SB, family S8, subfamily C).
UR - http://www.scopus.com/inward/record.url?scp=22344448312&partnerID=8YFLogxK
M3 - Article
C2 - 15954613
AN - SCOPUS:22344448312
SN - 1373-7503
VL - 66
SP - 329
EP - 332
JO - Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)
JF - Mededelingen (Rijksuniversiteit te Gent. Fakulteit van de Landbouwkundige en Toegepaste Biologische Wetenschappen)
IS - 3 a
ER -