Protein microarray detection strategies: Focus on direct detection technologies

Virginia Espina; Elisa C. Woodhouse; Julia Wulfkuhle; Heather D. Asmussen; Emanuel F. Petricoin; Lance A. Liotta

doi:10.1016/j.jim.2004.04.013

Protein microarray detection strategies: Focus on direct detection technologies

Virginia Espina^*, Elisa C. Woodhouse, Julia Wulfkuhle, Heather D. Asmussen, Emanuel F. Petricoin, Lance A. Liotta

^*Corresponding author for this work

Research output: Contribution to journal › Review article › peer-review

179 Scopus citations

Abstract

Protein microarrays are being utilized for functional proteomic analysis, providing information not obtainable by gene arrays. Microarray technology is applicable for studying protein-protein, protein-ligand, kinase activity and posttranslational modifications of proteins. A precise and sensitive protein microarray, the direct detection or reverse-phase microarray, has been applied to ongoing clinical trials at the National Cancer Institute for studying phosphorylation events in EGF-receptor-mediated cell signaling pathways. The variety of microarray applications allows for multiple, creative microarray designs and detection strategies. Herein, we discuss detection strategies and challenges for protein microarray technology, focusing on direct detection of protein microarrays.

Original language	English
Pages (from-to)	121-133
Number of pages	13
Journal	Journal of Immunological Methods
Volume	290
Issue number	1-2
DOIs	https://doi.org/10.1016/j.jim.2004.04.013
State	Published - Jul 2004
Externally published	Yes

Keywords

Catalyzed reporter deposition
Chromogens
Fluorescence
Molecular profiling
Protein microarray

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title = "Protein microarray detection strategies: Focus on direct detection technologies",

abstract = "Protein microarrays are being utilized for functional proteomic analysis, providing information not obtainable by gene arrays. Microarray technology is applicable for studying protein-protein, protein-ligand, kinase activity and posttranslational modifications of proteins. A precise and sensitive protein microarray, the direct detection or reverse-phase microarray, has been applied to ongoing clinical trials at the National Cancer Institute for studying phosphorylation events in EGF-receptor-mediated cell signaling pathways. The variety of microarray applications allows for multiple, creative microarray designs and detection strategies. Herein, we discuss detection strategies and challenges for protein microarray technology, focusing on direct detection of protein microarrays.",

keywords = "Catalyzed reporter deposition, Chromogens, Fluorescence, Molecular profiling, Protein microarray",

author = "Virginia Espina and Woodhouse, {Elisa C.} and Julia Wulfkuhle and Asmussen, {Heather D.} and Petricoin, {Emanuel F.} and Liotta, {Lance A.}",

year = "2004",

month = jul,

doi = "10.1016/j.jim.2004.04.013",

language = "English",

volume = "290",

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journal = "Journal of Immunological Methods",

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T1 - Protein microarray detection strategies

T2 - Focus on direct detection technologies

AU - Espina, Virginia

AU - Woodhouse, Elisa C.

AU - Wulfkuhle, Julia

AU - Asmussen, Heather D.

AU - Petricoin, Emanuel F.

AU - Liotta, Lance A.

PY - 2004/7

Y1 - 2004/7

N2 - Protein microarrays are being utilized for functional proteomic analysis, providing information not obtainable by gene arrays. Microarray technology is applicable for studying protein-protein, protein-ligand, kinase activity and posttranslational modifications of proteins. A precise and sensitive protein microarray, the direct detection or reverse-phase microarray, has been applied to ongoing clinical trials at the National Cancer Institute for studying phosphorylation events in EGF-receptor-mediated cell signaling pathways. The variety of microarray applications allows for multiple, creative microarray designs and detection strategies. Herein, we discuss detection strategies and challenges for protein microarray technology, focusing on direct detection of protein microarrays.

AB - Protein microarrays are being utilized for functional proteomic analysis, providing information not obtainable by gene arrays. Microarray technology is applicable for studying protein-protein, protein-ligand, kinase activity and posttranslational modifications of proteins. A precise and sensitive protein microarray, the direct detection or reverse-phase microarray, has been applied to ongoing clinical trials at the National Cancer Institute for studying phosphorylation events in EGF-receptor-mediated cell signaling pathways. The variety of microarray applications allows for multiple, creative microarray designs and detection strategies. Herein, we discuss detection strategies and challenges for protein microarray technology, focusing on direct detection of protein microarrays.

KW - Catalyzed reporter deposition

KW - Chromogens

KW - Fluorescence

KW - Molecular profiling

KW - Protein microarray

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DO - 10.1016/j.jim.2004.04.013

M3 - Review article

C2 - 15261576

AN - SCOPUS:3142714383

SN - 0022-1759

VL - 290

SP - 121

EP - 133

JO - Journal of Immunological Methods

JF - Journal of Immunological Methods

IS - 1-2

ER -

Protein microarray detection strategies: Focus on direct detection technologies

Abstract

Keywords

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