Protein microarrays: Molecular profiling technologies for clinical specimens

Virginia Espina*, Arpita I. Mehta, Mary E. Winters, Valerie Calvert, Julia Wulfkuhle, Emanuel F. Petricoin, Lance A. Liotta

*Corresponding author for this work

Research output: Contribution to journalReview articlepeer-review

215 Scopus citations


Proteomics, the study of protein function within biologic systems, will further our understanding of cancer pathogenesis. Coupled with transcript profiling, proteomics can herald the advent of molecular therapy tailored to the individual patient's neoplasm. Protein microarrays, one emerging class of proteomic technologies, have broad applications for discovery and quantitative analysis. This technology is uniquely suited to gather information about the post-translational modifications of proteins reflecting the activity state of signal pathways and networks. Protein microarrays now make it feasible to conduct signal network profiling within cellular samples. Nevertheless, to be successful, design and use of protein microarrays must take into consideration enormous analytical challenges. A subclass of protein microarrays, Reverse Phase Arrays, created to meet these challenges, has been optimized for use with tissue specimens, and is now in use for the analysis of biopsy samples for clinical trial research.

Original languageEnglish
Pages (from-to)2091-2100
Number of pages10
Issue number11
StatePublished - Nov 2003
Externally publishedYes


  • Catalyzed reporter deposition
  • Individual targeted therapy
  • Laser capture microdissection
  • Molecular profiling
  • Protein microarray
  • Review


Dive into the research topics of 'Protein microarrays: Molecular profiling technologies for clinical specimens'. Together they form a unique fingerprint.

Cite this