TY - JOUR
T1 - Protein pyrophosphorylation by diphosphoinositol pentakisphosphate (InsP7).
AU - Werner, J. Kent
AU - Speed, Traci
AU - Bhandari, Rashna
PY - 2010
Y1 - 2010
N2 - Diphosphoinositol polyphosphates, also known as inositol pyrophosphates, are a family of water soluble inositol phosphates that possess diphosphate or pyrophosphate moieties. In the presence of divalent cations such as Mg(2+), the "high energy" beta phosphate can be transferred from the inositol pyrophosphates, InsP(7) and InsP(8), to prephosphorylated serine residues on proteins, to form pyrophosphoserine. This chapter provides detailed methods to identify proteins that are substrates for pyrophosphorylation by InsP(7), conduct phosphorylation assays on purified protein, and detect protein pyrophosphorylation.
AB - Diphosphoinositol polyphosphates, also known as inositol pyrophosphates, are a family of water soluble inositol phosphates that possess diphosphate or pyrophosphate moieties. In the presence of divalent cations such as Mg(2+), the "high energy" beta phosphate can be transferred from the inositol pyrophosphates, InsP(7) and InsP(8), to prephosphorylated serine residues on proteins, to form pyrophosphoserine. This chapter provides detailed methods to identify proteins that are substrates for pyrophosphorylation by InsP(7), conduct phosphorylation assays on purified protein, and detect protein pyrophosphorylation.
UR - http://www.scopus.com/inward/record.url?scp=78049389339&partnerID=8YFLogxK
U2 - 10.1007/978-1-60327-175-2_6
DO - 10.1007/978-1-60327-175-2_6
M3 - Article
C2 - 20645183
AN - SCOPUS:78049389339
SN - 1064-3745
VL - 645
SP - 87
EP - 102
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -