Protein pyrophosphorylation by diphosphoinositol pentakisphosphate (InsP7).

J. Kent Werner; Traci Speed; Rashna Bhandari

doi:10.1007/978-1-60327-175-2_6

Protein pyrophosphorylation by diphosphoinositol pentakisphosphate (InsP7).

J. Kent Werner, Traci Speed, Rashna Bhandari

Neurology

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

Diphosphoinositol polyphosphates, also known as inositol pyrophosphates, are a family of water soluble inositol phosphates that possess diphosphate or pyrophosphate moieties. In the presence of divalent cations such as Mg(2+), the "high energy" beta phosphate can be transferred from the inositol pyrophosphates, InsP(7) and InsP(8), to prephosphorylated serine residues on proteins, to form pyrophosphoserine. This chapter provides detailed methods to identify proteins that are substrates for pyrophosphorylation by InsP(7), conduct phosphorylation assays on purified protein, and detect protein pyrophosphorylation.

Original language	English
Pages (from-to)	87-102
Number of pages	16
Journal	Methods in molecular biology (Clifton, N.J.)
Volume	645
DOIs	https://doi.org/10.1007/978-1-60327-175-2_6
State	Published - 2010

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title = "Protein pyrophosphorylation by diphosphoinositol pentakisphosphate (InsP7).",

abstract = "Diphosphoinositol polyphosphates, also known as inositol pyrophosphates, are a family of water soluble inositol phosphates that possess diphosphate or pyrophosphate moieties. In the presence of divalent cations such as Mg(2+), the {"}high energy{"} beta phosphate can be transferred from the inositol pyrophosphates, InsP(7) and InsP(8), to prephosphorylated serine residues on proteins, to form pyrophosphoserine. This chapter provides detailed methods to identify proteins that are substrates for pyrophosphorylation by InsP(7), conduct phosphorylation assays on purified protein, and detect protein pyrophosphorylation.",

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AU - Speed, Traci

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AB - Diphosphoinositol polyphosphates, also known as inositol pyrophosphates, are a family of water soluble inositol phosphates that possess diphosphate or pyrophosphate moieties. In the presence of divalent cations such as Mg(2+), the "high energy" beta phosphate can be transferred from the inositol pyrophosphates, InsP(7) and InsP(8), to prephosphorylated serine residues on proteins, to form pyrophosphoserine. This chapter provides detailed methods to identify proteins that are substrates for pyrophosphorylation by InsP(7), conduct phosphorylation assays on purified protein, and detect protein pyrophosphorylation.

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JF - Methods in molecular biology (Clifton, N.J.)

ER -

Protein pyrophosphorylation by diphosphoinositol pentakisphosphate (InsP7).

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