Proteolipidic composition of exosomes changes during reticulocyte maturation

Kévin Carayon*, Karima Chaoui, Elsa Ronzier, Ikrame Lazar, Justine Bertrand-Michel, Véronique Roques, Stéphanie Balor, François Terce, André Lopez, Laurence Salomé, Etienne Joly

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

152 Scopus citations


During the orchestrated process leading to mature erythrocytes, reticulocytes must synthesize large amounts of hemoglobin, while eliminating numerous cellular components. Exosomes are small secreted vesicles that play an important role in this process of specific elimination. To understand the mechanisms of proteolipidic sorting leading to their biogenesis, we have explored changes in the composition of exosomes released by reticulocytes during their differentiation, in parallel to their physical properties. By combining proteomic and lipidomic approaches, we found dramatic alterations in the composition of the exosomes retrieved over the course of a 7-day in vitro differentiation protocol. Our data support a previously proposed model, whereby in reticulocytes the biogenesis of exosomes involves several distinct mechanisms for the preferential recruitment of particular proteins and lipids and suggest that the respective prominence of those pathways changes over the course of the differentiation process.

Original languageEnglish
Pages (from-to)34426-34439
Number of pages14
JournalJournal of Biological Chemistry
Issue number39
StatePublished - 30 Oct 2011
Externally publishedYes


Dive into the research topics of 'Proteolipidic composition of exosomes changes during reticulocyte maturation'. Together they form a unique fingerprint.

Cite this