Proteomic analysis of human prostate cancer

Mamoun Ahram; Carolyn J.M. Best; Michael J. Flaig; John W. Gillespie; Isabel M. Leiva; Rodrigo F. Chuaqui; Ge Zhou; Hungjun Shu; Paul H. Duray; W. Marston Linehan; Mark Raffeld; David K. Ornstein; Yingming Zhao; Emanuel F. Petricoin; Michael R. Emmert-Buck

doi:10.1002/mc.10019

Proteomic analysis of human prostate cancer

Mamoun Ahram, Carolyn J.M. Best, Michael J. Flaig, John W. Gillespie, Isabel M. Leiva, Rodrigo F. Chuaqui, Ge Zhou, Hungjun Shu, Paul H. Duray, W. Marston Linehan, Mark Raffeld, David K. Ornstein, Yingming Zhao, Emanuel F. Petricoin, Michael R. Emmert-Buck^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

131 Scopus citations

Abstract

Proteomics is a promising approach in the identification of proteins and biochemical pathways involved in tumorigenesis. In an effort to discover such proteins and pathways that are deregulated in prostate tumorigenesis, cellular proteomes of matched normal prostate epithelial cells and high-grade prostate cancer cells were analyzed by tissue microdissection, two-dimensional electrophoresis, and mass spectrometry. Forty protein alterations were detected in the tumors; however, the majority of these changes were not shared among the 12 neoplasms. In contrast, parallel cDNA microarray analysis identified a number of common gene expression changes. The marked heterogeneity of the observed protein alterations may have significance with regard to tumor biology and research strategies for molecular profiling analyses of human prostate cancer.

Original language	English
Pages (from-to)	9-15
Number of pages	7
Journal	Molecular Carcinogenesis
Volume	33
Issue number	1
DOIs	https://doi.org/10.1002/mc.10019
State	Published - 2002
Externally published	Yes

Keywords

Heterogeneity
Microarray
Microdissection
Prostate cancer
Proteomics

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title = "Proteomic analysis of human prostate cancer",

abstract = "Proteomics is a promising approach in the identification of proteins and biochemical pathways involved in tumorigenesis. In an effort to discover such proteins and pathways that are deregulated in prostate tumorigenesis, cellular proteomes of matched normal prostate epithelial cells and high-grade prostate cancer cells were analyzed by tissue microdissection, two-dimensional electrophoresis, and mass spectrometry. Forty protein alterations were detected in the tumors; however, the majority of these changes were not shared among the 12 neoplasms. In contrast, parallel cDNA microarray analysis identified a number of common gene expression changes. The marked heterogeneity of the observed protein alterations may have significance with regard to tumor biology and research strategies for molecular profiling analyses of human prostate cancer.",

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author = "Mamoun Ahram and Best, {Carolyn J.M.} and Flaig, {Michael J.} and Gillespie, {John W.} and Leiva, {Isabel M.} and Chuaqui, {Rodrigo F.} and Ge Zhou and Hungjun Shu and Duray, {Paul H.} and Linehan, {W. Marston} and Mark Raffeld and Ornstein, {David K.} and Yingming Zhao and Petricoin, {Emanuel F.} and Emmert-Buck, {Michael R.}",

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doi = "10.1002/mc.10019",

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T1 - Proteomic analysis of human prostate cancer

AU - Ahram, Mamoun

AU - Best, Carolyn J.M.

AU - Flaig, Michael J.

AU - Gillespie, John W.

AU - Leiva, Isabel M.

AU - Chuaqui, Rodrigo F.

AU - Zhou, Ge

AU - Shu, Hungjun

AU - Duray, Paul H.

AU - Linehan, W. Marston

AU - Raffeld, Mark

AU - Ornstein, David K.

AU - Zhao, Yingming

AU - Petricoin, Emanuel F.

AU - Emmert-Buck, Michael R.

PY - 2002

Y1 - 2002

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AB - Proteomics is a promising approach in the identification of proteins and biochemical pathways involved in tumorigenesis. In an effort to discover such proteins and pathways that are deregulated in prostate tumorigenesis, cellular proteomes of matched normal prostate epithelial cells and high-grade prostate cancer cells were analyzed by tissue microdissection, two-dimensional electrophoresis, and mass spectrometry. Forty protein alterations were detected in the tumors; however, the majority of these changes were not shared among the 12 neoplasms. In contrast, parallel cDNA microarray analysis identified a number of common gene expression changes. The marked heterogeneity of the observed protein alterations may have significance with regard to tumor biology and research strategies for molecular profiling analyses of human prostate cancer.

KW - Heterogeneity

KW - Microarray

KW - Microdissection

KW - Prostate cancer

KW - Proteomics

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JO - Molecular Carcinogenesis

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Proteomic analysis of human prostate cancer

Abstract

Keywords

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