Proteomic analysis of human prostate cancer

Mamoun Ahram, Carolyn J.M. Best, Michael J. Flaig, John W. Gillespie, Isabel M. Leiva, Rodrigo F. Chuaqui, Ge Zhou, Hungjun Shu, Paul H. Duray, W. Marston Linehan, Mark Raffeld, David K. Ornstein, Yingming Zhao, Emanuel F. Petricoin, Michael R. Emmert-Buck*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

130 Scopus citations


Proteomics is a promising approach in the identification of proteins and biochemical pathways involved in tumorigenesis. In an effort to discover such proteins and pathways that are deregulated in prostate tumorigenesis, cellular proteomes of matched normal prostate epithelial cells and high-grade prostate cancer cells were analyzed by tissue microdissection, two-dimensional electrophoresis, and mass spectrometry. Forty protein alterations were detected in the tumors; however, the majority of these changes were not shared among the 12 neoplasms. In contrast, parallel cDNA microarray analysis identified a number of common gene expression changes. The marked heterogeneity of the observed protein alterations may have significance with regard to tumor biology and research strategies for molecular profiling analyses of human prostate cancer.

Original languageEnglish
Pages (from-to)9-15
Number of pages7
JournalMolecular Carcinogenesis
Issue number1
StatePublished - 2002
Externally publishedYes


  • Heterogeneity
  • Microarray
  • Microdissection
  • Prostate cancer
  • Proteomics


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