Proteomic analysis of mouse cortical neurons by isotope-coded affinity tags and mass spectrometry

Li Rong Yu; Mark D. Johnson; Thomas P. Conrads; Sang Won Lee; Ronald J. Moore; Nikola Tolic; Richard S. Morrison; Timothy D. Veenstra; Richard D. Smith

Proteomic analysis of mouse cortical neurons by isotope-coded affinity tags and mass spectrometry

Li Rong Yu^*, Mark D. Johnson, Thomas P. Conrads, Sang Won Lee, Ronald J. Moore, Nikola Tolic, Richard S. Morrison, Timothy D. Veenstra, Richard D. Smith

^*Corresponding author for this work

Research output: Contribution to conference › Paper › peer-review

Abstract

The role of isotope coded affinity tags (ICAT) and mass spectrometry to measure protein expression changes between untreated and camptothecintreated mouse cortical neurons, was discussed. Equivalent amounts of protein extracted from the control and camptothecin-treated cortical neurons were labeled with the 'light' (ICAT-D ₀) and the heavy (ICAT-D ₈) isotopic version of the ICAT reagent. The sample was measured by cLC-Fourier transform ion cyclotron resonance mass spectrometry. More than 2,300 peptide pairs were detected in the FTICR-MS analysis.

Original language	English
Pages	281-282
Number of pages	2
State	Published - 2002
Externally published	Yes
Event	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics - Orlando, FL, United States Duration: 2 Jun 2002 → 6 Jun 2002

Conference

Conference	Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics
Country/Territory	United States
City	Orlando, FL
Period	2/06/02 → 6/06/02

Cite this

@conference{270a201034904611921a2c5eb5bdb346,

title = "Proteomic analysis of mouse cortical neurons by isotope-coded affinity tags and mass spectrometry",

abstract = "The role of isotope coded affinity tags (ICAT) and mass spectrometry to measure protein expression changes between untreated and camptothecintreated mouse cortical neurons, was discussed. Equivalent amounts of protein extracted from the control and camptothecin-treated cortical neurons were labeled with the 'light' (ICAT-D 0) and the heavy (ICAT-D 8) isotopic version of the ICAT reagent. The sample was measured by cLC-Fourier transform ion cyclotron resonance mass spectrometry. More than 2,300 peptide pairs were detected in the FTICR-MS analysis.",

author = "Yu, {Li Rong} and Johnson, {Mark D.} and Conrads, {Thomas P.} and Lee, {Sang Won} and Moore, {Ronald J.} and Nikola Tolic and Morrison, {Richard S.} and Veenstra, {Timothy D.} and Smith, {Richard D.}",

year = "2002",

language = "English",

pages = "281--282",

note = "Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics ; Conference date: 02-06-2002 Through 06-06-2002",

}

TY - CONF

T1 - Proteomic analysis of mouse cortical neurons by isotope-coded affinity tags and mass spectrometry

AU - Yu, Li Rong

AU - Johnson, Mark D.

AU - Conrads, Thomas P.

AU - Lee, Sang Won

AU - Moore, Ronald J.

AU - Tolic, Nikola

AU - Morrison, Richard S.

AU - Veenstra, Timothy D.

AU - Smith, Richard D.

PY - 2002

Y1 - 2002

N2 - The role of isotope coded affinity tags (ICAT) and mass spectrometry to measure protein expression changes between untreated and camptothecintreated mouse cortical neurons, was discussed. Equivalent amounts of protein extracted from the control and camptothecin-treated cortical neurons were labeled with the 'light' (ICAT-D 0) and the heavy (ICAT-D 8) isotopic version of the ICAT reagent. The sample was measured by cLC-Fourier transform ion cyclotron resonance mass spectrometry. More than 2,300 peptide pairs were detected in the FTICR-MS analysis.

AB - The role of isotope coded affinity tags (ICAT) and mass spectrometry to measure protein expression changes between untreated and camptothecintreated mouse cortical neurons, was discussed. Equivalent amounts of protein extracted from the control and camptothecin-treated cortical neurons were labeled with the 'light' (ICAT-D 0) and the heavy (ICAT-D 8) isotopic version of the ICAT reagent. The sample was measured by cLC-Fourier transform ion cyclotron resonance mass spectrometry. More than 2,300 peptide pairs were detected in the FTICR-MS analysis.

UR - http://www.scopus.com/inward/record.url?scp=2442691654&partnerID=8YFLogxK

M3 - Paper

AN - SCOPUS:2442691654

SP - 281

EP - 282

T2 - Proceedings - 50th ASMS Conference on Mass Spectrometry and Allied Topics

Y2 - 2 June 2002 through 6 June 2002

ER -

Proteomic analysis of mouse cortical neurons by isotope-coded affinity tags and mass spectrometry

Abstract

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