Proteomic analysis of pancreatic ductal adenocarcinoma cells reveals metabolic alterations

Weidong Zhou*, Michela Capello, Claudia Fredolini, Lorenzo Piemonti, Lance A. Liotta, Francesco Novelli, Emanuel F. Petricoin

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


In this present work, we characterized the proteomes of pancreatic ductal adenocarcinoma (PDAC) cells and normal pancreatic duct cells by mass spectrometry using LTQ-Orbitrap, and identified more than 1200 proteins from each sample. On the basis of spectra count label-free quantification approach, we identified a large number of differentially expressed metabolic enzymes and proteins involved in cytoskeleton, cell adhesion, transport, transcription, translation, and cell proliferation as well. The data demonstrated that metabolic pathways were altered in PDAC, consistent with Warburg effect. Our analysis provides a potentially comprehensive picture of metabolism in PDAC, which may serve as the basis of new diagnostic and treatment of PDAC.

Original languageEnglish
Pages (from-to)1944-1952
Number of pages9
JournalJournal of Proteome Research
Issue number4
StatePublished - 1 Apr 2011
Externally publishedYes


  • LTQ-Orbitrap
  • Warburg effect
  • mass spectrometry
  • metabolism
  • pancreatic ductal adenocarcinoma
  • proteomics


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