Proteomic analysis of pancreatic ductal adenocarcinoma cells reveals metabolic alterations

Weidong Zhou; Michela Capello; Claudia Fredolini; Lorenzo Piemonti; Lance A. Liotta; Francesco Novelli; Emanuel F. Petricoin

doi:10.1021/pr101179t

Proteomic analysis of pancreatic ductal adenocarcinoma cells reveals metabolic alterations

Weidong Zhou^*, Michela Capello, Claudia Fredolini, Lorenzo Piemonti, Lance A. Liotta, Francesco Novelli, Emanuel F. Petricoin

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

In this present work, we characterized the proteomes of pancreatic ductal adenocarcinoma (PDAC) cells and normal pancreatic duct cells by mass spectrometry using LTQ-Orbitrap, and identified more than 1200 proteins from each sample. On the basis of spectra count label-free quantification approach, we identified a large number of differentially expressed metabolic enzymes and proteins involved in cytoskeleton, cell adhesion, transport, transcription, translation, and cell proliferation as well. The data demonstrated that metabolic pathways were altered in PDAC, consistent with Warburg effect. Our analysis provides a potentially comprehensive picture of metabolism in PDAC, which may serve as the basis of new diagnostic and treatment of PDAC.

Original language	English
Pages (from-to)	1944-1952
Number of pages	9
Journal	Journal of Proteome Research
Volume	10
Issue number	4
DOIs	https://doi.org/10.1021/pr101179t
State	Published - 1 Apr 2011
Externally published	Yes

Keywords

LTQ-Orbitrap
Warburg effect
mass spectrometry
metabolism
pancreatic ductal adenocarcinoma
proteomics

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10.1021/pr101179t

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title = "Proteomic analysis of pancreatic ductal adenocarcinoma cells reveals metabolic alterations",

abstract = "In this present work, we characterized the proteomes of pancreatic ductal adenocarcinoma (PDAC) cells and normal pancreatic duct cells by mass spectrometry using LTQ-Orbitrap, and identified more than 1200 proteins from each sample. On the basis of spectra count label-free quantification approach, we identified a large number of differentially expressed metabolic enzymes and proteins involved in cytoskeleton, cell adhesion, transport, transcription, translation, and cell proliferation as well. The data demonstrated that metabolic pathways were altered in PDAC, consistent with Warburg effect. Our analysis provides a potentially comprehensive picture of metabolism in PDAC, which may serve as the basis of new diagnostic and treatment of PDAC.",

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AU - Zhou, Weidong

AU - Capello, Michela

AU - Fredolini, Claudia

AU - Piemonti, Lorenzo

AU - Liotta, Lance A.

AU - Novelli, Francesco

AU - Petricoin, Emanuel F.

PY - 2011/4/1

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AB - In this present work, we characterized the proteomes of pancreatic ductal adenocarcinoma (PDAC) cells and normal pancreatic duct cells by mass spectrometry using LTQ-Orbitrap, and identified more than 1200 proteins from each sample. On the basis of spectra count label-free quantification approach, we identified a large number of differentially expressed metabolic enzymes and proteins involved in cytoskeleton, cell adhesion, transport, transcription, translation, and cell proliferation as well. The data demonstrated that metabolic pathways were altered in PDAC, consistent with Warburg effect. Our analysis provides a potentially comprehensive picture of metabolism in PDAC, which may serve as the basis of new diagnostic and treatment of PDAC.

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Proteomic analysis of pancreatic ductal adenocarcinoma cells reveals metabolic alterations

Abstract

Keywords

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