Proteomic and biochemical analysis of purified human immunodeficiency virus type 1 produced from infected monocyte-derived macrophages

Elena Chertova*, Oleg Chertov, Lori V. Coren, James D. Roser, Charles M. Trubey, Julian W. Bess, Raymond C. Sowder, Eugene Barsov, Brian L. Hood, Robert J. Fisher, Kunio Nagashima, Thomas P. Conrads, Timothy D. Veenstra, Jeffrey D. Lifson, David E. Ott

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

373 Scopus citations

Abstract

Human immunodeficiency virus type 1 (HIV-1) infects CD4+ T lymphocytes and monocytes/macrophages, incorporating host proteins in the process of assembly and budding. Analysis of the host cell proteins incorporated into virions can provide insights into viral biology. We characterized proteins in highly purified HIV-1 virions produced from human monocyte-derived macrophages (MDM), within which virus buds predominantly into intracytoplasmic vesicles, in contrast to the plasmalemmal budding of HIV-1 typically seen with infected T cells. Liquid chromatography-linked tandem mass spectrometry of highly purified virions identified many cellular proteins, including 33 previously described proteins in HIV-1 preparations from other cell types. Proteins involved in many different cellular structures and functions were present, including those from the cytoskeleton, adhesion, signaling, intracellular trafficking, chaperone, metabolic, ubiquitin/proteasomal, and immune response systems. We also identified annexins, annexin-binding proteins, Rab proteins, and other proteins involved in membrane organization, vesicular trafficking, and late endosomal function, as well as apolipoprotein E, which participates in cholesterol transport, immunoregulation, and modulation of cell growth and differentiation. Several tetraspanins, markers of the late endosomal compartment, were also identified. MDM-derived HIV contained 26 of 37 proteins previously found in exosomes, consistent with the idea that HIV uses the late endosome/multivesicular body pathway during virion budding from macrophages.

Original languageEnglish
Pages (from-to)9039-9052
Number of pages14
JournalJournal of Virology
Volume80
Issue number18
DOIs
StatePublished - Sep 2006
Externally publishedYes

Fingerprint

Dive into the research topics of 'Proteomic and biochemical analysis of purified human immunodeficiency virus type 1 produced from infected monocyte-derived macrophages'. Together they form a unique fingerprint.

Cite this