@article{2440a3a75f804f80af044e46d1dc7d53,
title = "Proteomic sample preparation for blast wound characterization",
abstract = "Background: Blast wounds often involve diverse tissue types and require substantial time and treatment for appropriate healing. Some of these subsequent wounds become colonized with bacteria requiring a better understanding of how the host responds to these bacteria and what proteomic factors contribute wound healing outcome. In addition, using reliable and effective proteomic sample preparation procedures can lead to novel biomarkers for improved diagnosis and therapy.Results: To address this need, suitable sample preparation for 2-D DIGE proteomic characterization of wound effluent and serum samples from combat-wounded patients was investigated. Initial evaluation of crude effluent and serum proved the necessity of high abundant protein depletion. Subsequently, both samples were successfully depleted using Agilent Multiple Affinity Removal system and showed greatly improved 2-D spot maps, comprising 1,800 and 1,200 protein spots, respectively.Conclusion: High abundant protein removal was necessary for both wound effluent and serum. This is the first study to show a successful method for high abundant protein depletion from wound effluent which is compatible with downstream 2-D DIGE analysis. This development allows for improved biomarker discovery in wound effluent and serum samples.",
keywords = "2-D DIGE, Biomarker discovery, Blast wound, High abundant protein removal, Proteomics, Serum, Wound effluent",
author = "Chromy, {Brett A.} and Angela Eldridge and Forsberg, {Jonathan A.} and Brown, {Trevor S.} and Kirkup, {Benjamin C.} and Eric Elster and Paul Luciw",
note = "Funding Information: The views expressed in this manuscript are those of the authors and do not reflect the official policy of the Department of the Army, Department of the Navy, the Department of Defense or the United States Government. This effort was supported (in part) by the U.S. Navy Bureau of Medicine and Surgery under the Medical Development Program and Office of Naval Research. Several co-authors are military service members (or employee of the U.S. Government). This work was prepared as part of their official duties. Title 17 U.S.C. 105 provides the “Copyright protection under this title is not available for any work of the United States Government.” Title 17 U.S.C. 101 defines a U.S. Government work as a work prepared by a military service member or employee of the U.S. Government as part of that person{\textquoteright}s official duties. I/We certify that all individuals who qualify as authors have been listed; each has participated in the conception and design of this work, the analysis of data (when applicable), the writing of the document, and the approval of the submission of this version; that the document represents valid work; that if we used information derived from another source, we obtained all necessary approvals to use it and made appropriate acknowledgements in the document; and that each takes public responsibility for it. Funding Information: The project depicted was sponsored by the U.S. Army Medical Research and Materiel Command (Award number: MIPR1EO89M1115). The U.S. Army Medical Research Acquisition Activity (820 Chandler Street, Fort Detrick MD 21702–5014) is the awarding and administering acquisition office. Partial funding for this project was also provided by the Proteomics Initiative of the Department of Pathology and Laboratory Medicine at U.C. Davis. We gratefully acknowledge Crystal Jaing, Nicholas Be, and Ray Lenhoff for very helpful discussions. The opinions or assertions contained herein are the private views of the authors, and are not to be construed as official, or as reflecting the position or policy of the government, including the Departments of the Army, Navy, or Defense.",
year = "2014",
month = feb,
day = "14",
doi = "10.1186/1477-5956-12-10",
language = "English",
volume = "12",
journal = "Proteome Science",
issn = "1477-5956",
number = "1",
}