Proteomics based selection achieves complete response to HER2 therapy in HER2 IHC 0 breast cancer

Laura E. Johnston, Jamie Randall, Safae Chouraichi, Mary Luu, Allison L. Hunt, Lauren Mauro, Claudius Mueller, Justin B. Davis, Emanuel F. Petricoin, Thomas P. Conrads, Timothy L. Cannon, Jasmine Huynh*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

Abstract

Recent trials have shown the efficacy of trastuzumab deruxtecan (T-DXd) in HER2-negative patients, but there is not yet a way to identify which patients will best respond, especially with the inability of current HER2 IHC and FISH assays to accurately determine HER2 expression in the unamplified setting. Here, we present a heavily pre-treated patient with triple-negative breast cancer (HER2 IHC 0 who had a complete response to T-DXd. In this case, we used a CLIA-certified reverse-phase protein array-based proteomic assay (RPPA) to determine that the patient had moderate HER2 protein expression (HER2Total 2+, 42%) and activation (HER2Y1248 1+, 23%). Using these results, we determined that the patient may benefit from T-Dxd despite being traditionally qualified as HER2 IHC 0. These findings highlight the potential for proteomics-based assays that may more accurately quantitate HER2 and (its activation) in the HER2 unamplified/IHC 0 setting to better select patients whose tumors are classically molecularly defined as HER2 IHC 0, but still could respond to HER2-directed therapy, and give patients access to therapies which for which they otherwise would not be eligible.

Original languageEnglish
Article number203
Journalnpj Precision Oncology
Volume8
Issue number1
DOIs
StatePublished - Dec 2024
Externally publishedYes

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