Purification of antigenized immunoglobulins derivatized with monomethoxypolyethylene glycol

T. D. Brumeanu; H. Zaghouani; C. Bona

doi:10.1016/0021-9673(94)01275-J

Purification of antigenized immunoglobulins derivatized with monomethoxypolyethylene glycol

T. D. Brumeanu, H. Zaghouani, C. Bona^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Genetically engineered immunoglobulins (Igs) carrying viral B or T cell peptides in the CDR3 loop, function as efficient delivery system of the defined viral epitopes. Two of these antigenized Igs (AIgs) were derivatized with 2-O-monomethoxypolyethylene glycol-4,6-dichloro-s-triazine (mPEG). Herein, we describe a two-step strategy to purify mPEG-derivatized AIgs (AIgs-mPEG). Unr chromatography using ammonium hydrogencarbonate as a buffer system. Mildly PEGylated AIgs were isolated from free and highly derivatized AIgs by anion-exchange chromatography. Electrophoretic analysis indicated that the AIgs-mPEG preparation contained less than 4·10^-4 M unreacted mPEG. This strategy may be applied to other mPEG-derivatized monoclonal antibodies.

Original language	English
Pages (from-to)	219-225
Number of pages	7
Journal	Journal of Chromatography A
Volume	696
Issue number	2
DOIs	https://doi.org/10.1016/0021-9673(94)01275-J
State	Published - 14 Apr 1995
Externally published	Yes

Access to Document

10.1016/0021-9673(94)01275-J

Cite this

@article{03c52a02a7e04b27a11b36dcb3c725aa,

title = "Purification of antigenized immunoglobulins derivatized with monomethoxypolyethylene glycol",

abstract = "Genetically engineered immunoglobulins (Igs) carrying viral B or T cell peptides in the CDR3 loop, function as efficient delivery system of the defined viral epitopes. Two of these antigenized Igs (AIgs) were derivatized with 2-O-monomethoxypolyethylene glycol-4,6-dichloro-s-triazine (mPEG). Herein, we describe a two-step strategy to purify mPEG-derivatized AIgs (AIgs-mPEG). Unr chromatography using ammonium hydrogencarbonate as a buffer system. Mildly PEGylated AIgs were isolated from free and highly derivatized AIgs by anion-exchange chromatography. Electrophoretic analysis indicated that the AIgs-mPEG preparation contained less than 4·10-4 M unreacted mPEG. This strategy may be applied to other mPEG-derivatized monoclonal antibodies.",

author = "Brumeanu, {T. D.} and H. Zaghouani and C. Bona",

year = "1995",

month = apr,

day = "14",

doi = "10.1016/0021-9673(94)01275-J",

language = "English",

volume = "696",

pages = "219--225",

journal = "Journal of Chromatography A",

issn = "0021-9673",

number = "2",

}

TY - JOUR

T1 - Purification of antigenized immunoglobulins derivatized with monomethoxypolyethylene glycol

AU - Brumeanu, T. D.

AU - Zaghouani, H.

AU - Bona, C.

PY - 1995/4/14

Y1 - 1995/4/14

N2 - Genetically engineered immunoglobulins (Igs) carrying viral B or T cell peptides in the CDR3 loop, function as efficient delivery system of the defined viral epitopes. Two of these antigenized Igs (AIgs) were derivatized with 2-O-monomethoxypolyethylene glycol-4,6-dichloro-s-triazine (mPEG). Herein, we describe a two-step strategy to purify mPEG-derivatized AIgs (AIgs-mPEG). Unr chromatography using ammonium hydrogencarbonate as a buffer system. Mildly PEGylated AIgs were isolated from free and highly derivatized AIgs by anion-exchange chromatography. Electrophoretic analysis indicated that the AIgs-mPEG preparation contained less than 4·10-4 M unreacted mPEG. This strategy may be applied to other mPEG-derivatized monoclonal antibodies.

AB - Genetically engineered immunoglobulins (Igs) carrying viral B or T cell peptides in the CDR3 loop, function as efficient delivery system of the defined viral epitopes. Two of these antigenized Igs (AIgs) were derivatized with 2-O-monomethoxypolyethylene glycol-4,6-dichloro-s-triazine (mPEG). Herein, we describe a two-step strategy to purify mPEG-derivatized AIgs (AIgs-mPEG). Unr chromatography using ammonium hydrogencarbonate as a buffer system. Mildly PEGylated AIgs were isolated from free and highly derivatized AIgs by anion-exchange chromatography. Electrophoretic analysis indicated that the AIgs-mPEG preparation contained less than 4·10-4 M unreacted mPEG. This strategy may be applied to other mPEG-derivatized monoclonal antibodies.

UR - http://www.scopus.com/inward/record.url?scp=0028955368&partnerID=8YFLogxK

U2 - 10.1016/0021-9673(94)01275-J

DO - 10.1016/0021-9673(94)01275-J

M3 - Article

C2 - 7538402

AN - SCOPUS:0028955368

SN - 0021-9673

VL - 696

SP - 219

EP - 225

JO - Journal of Chromatography A

JF - Journal of Chromatography A

IS - 2

ER -

Purification of antigenized immunoglobulins derivatized with monomethoxypolyethylene glycol

Abstract

Access to Document

Fingerprint

Cite this