Purification of antigenized immunoglobulins derivatized with monomethoxypolyethylene glycol

T. D. Brumeanu, H. Zaghouani, C. Bona*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

18 Scopus citations


Genetically engineered immunoglobulins (Igs) carrying viral B or T cell peptides in the CDR3 loop, function as efficient delivery system of the defined viral epitopes. Two of these antigenized Igs (AIgs) were derivatized with 2-O-monomethoxypolyethylene glycol-4,6-dichloro-s-triazine (mPEG). Herein, we describe a two-step strategy to purify mPEG-derivatized AIgs (AIgs-mPEG). Unr chromatography using ammonium hydrogencarbonate as a buffer system. Mildly PEGylated AIgs were isolated from free and highly derivatized AIgs by anion-exchange chromatography. Electrophoretic analysis indicated that the AIgs-mPEG preparation contained less than 4·10-4 M unreacted mPEG. This strategy may be applied to other mPEG-derivatized monoclonal antibodies.

Original languageEnglish
Pages (from-to)219-225
Number of pages7
JournalJournal of Chromatography A
Issue number2
StatePublished - 14 Apr 1995
Externally publishedYes


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