Purification of antigenized immunoglobulins derivatized with monomethoxypolyethylene glycol

T. D. Brumeanu; H. Zaghouani; C. Bona

doi:10.1016/0021-9673(94)01275-J

Purification of antigenized immunoglobulins derivatized with monomethoxypolyethylene glycol

T. D. Brumeanu, H. Zaghouani, C. Bona^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Genetically engineered immunoglobulins (Igs) carrying viral B or T cell peptides in the CDR3 loop, function as efficient delivery system of the defined viral epitopes. Two of these antigenized Igs (AIgs) were derivatized with 2-O-monomethoxypolyethylene glycol-4,6-dichloro-s-triazine (mPEG). Herein, we describe a two-step strategy to purify mPEG-derivatized AIgs (AIgs-mPEG). Unr chromatography using ammonium hydrogencarbonate as a buffer system. Mildly PEGylated AIgs were isolated from free and highly derivatized AIgs by anion-exchange chromatography. Electrophoretic analysis indicated that the AIgs-mPEG preparation contained less than 4·10^-4 M unreacted mPEG. This strategy may be applied to other mPEG-derivatized monoclonal antibodies.

Original language	English
Pages (from-to)	219-225
Number of pages	7
Journal	Journal of Chromatography A
Volume	696
Issue number	2
DOIs	https://doi.org/10.1016/0021-9673(94)01275-J
State	Published - 14 Apr 1995
Externally published	Yes

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title = "Purification of antigenized immunoglobulins derivatized with monomethoxypolyethylene glycol",

abstract = "Genetically engineered immunoglobulins (Igs) carrying viral B or T cell peptides in the CDR3 loop, function as efficient delivery system of the defined viral epitopes. Two of these antigenized Igs (AIgs) were derivatized with 2-O-monomethoxypolyethylene glycol-4,6-dichloro-s-triazine (mPEG). Herein, we describe a two-step strategy to purify mPEG-derivatized AIgs (AIgs-mPEG). Unr chromatography using ammonium hydrogencarbonate as a buffer system. Mildly PEGylated AIgs were isolated from free and highly derivatized AIgs by anion-exchange chromatography. Electrophoretic analysis indicated that the AIgs-mPEG preparation contained less than 4·10-4 M unreacted mPEG. This strategy may be applied to other mPEG-derivatized monoclonal antibodies.",

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AU - Brumeanu, T. D.

AU - Zaghouani, H.

AU - Bona, C.

PY - 1995/4/14

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N2 - Genetically engineered immunoglobulins (Igs) carrying viral B or T cell peptides in the CDR3 loop, function as efficient delivery system of the defined viral epitopes. Two of these antigenized Igs (AIgs) were derivatized with 2-O-monomethoxypolyethylene glycol-4,6-dichloro-s-triazine (mPEG). Herein, we describe a two-step strategy to purify mPEG-derivatized AIgs (AIgs-mPEG). Unr chromatography using ammonium hydrogencarbonate as a buffer system. Mildly PEGylated AIgs were isolated from free and highly derivatized AIgs by anion-exchange chromatography. Electrophoretic analysis indicated that the AIgs-mPEG preparation contained less than 4·10-4 M unreacted mPEG. This strategy may be applied to other mPEG-derivatized monoclonal antibodies.

AB - Genetically engineered immunoglobulins (Igs) carrying viral B or T cell peptides in the CDR3 loop, function as efficient delivery system of the defined viral epitopes. Two of these antigenized Igs (AIgs) were derivatized with 2-O-monomethoxypolyethylene glycol-4,6-dichloro-s-triazine (mPEG). Herein, we describe a two-step strategy to purify mPEG-derivatized AIgs (AIgs-mPEG). Unr chromatography using ammonium hydrogencarbonate as a buffer system. Mildly PEGylated AIgs were isolated from free and highly derivatized AIgs by anion-exchange chromatography. Electrophoretic analysis indicated that the AIgs-mPEG preparation contained less than 4·10-4 M unreacted mPEG. This strategy may be applied to other mPEG-derivatized monoclonal antibodies.

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Purification of antigenized immunoglobulins derivatized with monomethoxypolyethylene glycol

Abstract

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