Quantitative proteomics employing primary amine affinity tags

M. Van Hoang, Thomas P. Conrads, Timothy D. Veenstra, Josip Blonder, Atsushi Terunuma, Jonathan C. Vogel, Robert J. Fisher

Research output: Contribution to journalArticlepeer-review

22 Scopus citations


A proteomics-based method using stable isotope labeling to assess the relative abundance of peptides or proteins is described. Bradykinin and carbonic anhydrase were labeled with sulfosuccinimidyl-2-(biotinamido) ethyl-1,3-dithiopro-pionate, a membrane impermeant reagent that is reactive with primary amines. Specificity of the label to primary amines was demonstrated using tandem mass spectrometry. Also, relative quantitation was achieved by secondary labeling with natural isotopic abundance and stable isotope-labeled methyl iodide. We believe this to be an effective stable isotope-labeling method for quantitative proteomics.

Original languageEnglish
Pages (from-to)216-223
Number of pages8
JournalJournal of Biomolecular Techniques
Issue number3
StatePublished - Sep 2003
Externally publishedYes


  • Proteomics
  • Stable isotope labeling
  • Sulfo-NHS-SS-biotin


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