Raman spectroscopy and the spectral correlation index for predicting wound healing outcome: Towards in vivo application

Adam G. Berger, Nicole J. Crane, Eric A. Elster*

*Corresponding author for this work

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Combat wounds are sometimes confounded by healing complications that are not as prevalent in civilian wounds due to their high energy etiology. One complication of wound healing is dehiscence, where a surgically closed wound reopens after closure. This complication can have serious consequences for the patient, but knowledge about the molecular composition of the wound bed beyond what is readily visible may help clinicians mitigate these complications. It is necessary to develop techniques that can be used in vivo to assess and predict wound healing pointof-care so that care-takers can decide the best way to make informed clinical decisions regarding their patient's healing. Raman spectroscopy is a perfect candidate for predicting wound healing due to its ability to provide a detailed molecular fingerprint of the wound bed noninvasively. Here, we study the spectral correlation index, a measure of orthogonality, with ten reference tissue components to stratify wounds based on how they heal. We analyze these indexes over time to show the modulation of these tissue components over the wound healing process. Results show that qualitative observation of the spectra cannot reveal major differences between the dehisced and normal healing wounds, but the spectral correlation index can. Analysis of the spectral correlations across the wound healing process demonstrates the changes throughout the wound healing process, showing that early differences in tissue components may portend wound healing. Furthermore, Raman spectroscopy coupled with the spectral correlation index presents as a possible point-of-care tool for enabling discrimination of wounds with impaired healing.

Original languageEnglish
Title of host publicationOptical Diagnostics and Sensing XVI
Subtitle of host publicationToward Point-of-Care Diagnostics
EditorsGerard L. Cote
PublisherSPIE
ISBN (Electronic)9781628419498
DOIs
StatePublished - 2016
Externally publishedYes
EventOptical Diagnostics and Sensing XVI: Toward Point-of-Care Diagnostics - San Francisco, United States
Duration: 15 Feb 201616 Feb 2016

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume9715
ISSN (Print)1605-7422

Conference

ConferenceOptical Diagnostics and Sensing XVI: Toward Point-of-Care Diagnostics
Country/TerritoryUnited States
CitySan Francisco
Period15/02/1616/02/16

Keywords

  • Raman spectroscopy
  • combat-related trauma
  • spectral correlation index
  • spectral orthogonality
  • tissue components
  • wound bed
  • wound healing

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