Rapid and simultaneous detection of blaKPC and blaNDM by use of multiplex real-time PCR

Michael Milillo; Yoon I. Kwak; Erik Snesrud; Paige E. Waterman; Emil Lesho; Patrick McGann

doi:10.1128/JCM.03316-12

Rapid and simultaneous detection of bla_KPC and bla_NDM by use of multiplex real-time PCR

Michael Milillo, Yoon I. Kwak, Erik Snesrud, Paige E. Waterman, Emil Lesho, Patrick McGann^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

The increasing incidence of carbapenem nonsusceptibility among clinically important species is of global concern. Identification of the molecular mechanisms underlying carbapenem nonsusceptibility is critical for epidemiological investigations. In this report, we describe a real-time PCR-based assay capable of simultaneously detecting blaKPC and blaNDM, two of the most important carbapenemases, directly from culture in less than 90 min. The assay was validated with bla_KPC- and bla_NDM-carrying clinical isolates and demonstrated 100% concordance with the Carba NP test.

Original language	English
Pages (from-to)	1247-1249
Number of pages	3
Journal	Journal of Clinical Microbiology
Volume	51
Issue number	4
DOIs	https://doi.org/10.1128/JCM.03316-12
State	Published - Apr 2013
Externally published	Yes

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10.1128/JCM.03316-12

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abstract = "The increasing incidence of carbapenem nonsusceptibility among clinically important species is of global concern. Identification of the molecular mechanisms underlying carbapenem nonsusceptibility is critical for epidemiological investigations. In this report, we describe a real-time PCR-based assay capable of simultaneously detecting blaKPC and blaNDM, two of the most important carbapenemases, directly from culture in less than 90 min. The assay was validated with blaKPC- and blaNDM-carrying clinical isolates and demonstrated 100% concordance with the Carba NP test.",

author = "Michael Milillo and Kwak, {Yoon I.} and Erik Snesrud and Waterman, {Paige E.} and Emil Lesho and Patrick McGann",

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AU - Snesrud, Erik

AU - Waterman, Paige E.

AU - Lesho, Emil

AU - McGann, Patrick

PY - 2013/4

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AB - The increasing incidence of carbapenem nonsusceptibility among clinically important species is of global concern. Identification of the molecular mechanisms underlying carbapenem nonsusceptibility is critical for epidemiological investigations. In this report, we describe a real-time PCR-based assay capable of simultaneously detecting blaKPC and blaNDM, two of the most important carbapenemases, directly from culture in less than 90 min. The assay was validated with blaKPC- and blaNDM-carrying clinical isolates and demonstrated 100% concordance with the Carba NP test.

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IS - 4

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Rapid and simultaneous detection of blaKPC and blaNDM by use of multiplex real-time PCR

Abstract

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Rapid and simultaneous detection of bla_KPC and bla_NDM by use of multiplex real-time PCR