Rapid and simultaneous detection of the chlorhexidine and mupirocin resistance genes qacA/B and mupA in clinical isolates of methicillin-resistant Staphylococcus aureus

Patrick Mc Gann; Michael Milillo; Yoon I. Kwak; Reyes Quintero; Paige E. Waterman; Emil Lesho

doi:10.1016/j.diagmicrobio.2013.06.006

Rapid and simultaneous detection of the chlorhexidine and mupirocin resistance genes qacA/B and mupA in clinical isolates of methicillin-resistant Staphylococcus aureus

Patrick Mc Gann^*, Michael Milillo, Yoon I. Kwak, Reyes Quintero, Paige E. Waterman, Emil Lesho

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

28 Scopus citations

Abstract

We describe a real-time PCR-based assay capable of simultaneously detecting femA (Staphylococcus aureus-specific), mecA (methicillin resistance), qacA/B (chlorhexidine tolerance), and mupA (high-level mupirocin resistance) from bacterial cells in less than 90 minutes. The assay was validated with 1968 clinical MRSA submitted to a surveillance network.

Original language	English
Pages (from-to)	270-272
Number of pages	3
Journal	Diagnostic Microbiology and Infectious Disease
Volume	77
Issue number	3
DOIs	https://doi.org/10.1016/j.diagmicrobio.2013.06.006
State	Published - Nov 2013
Externally published	Yes

Keywords

Chlorhexidine
MRSA
MupA
Mupirocin
QacA

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10.1016/j.diagmicrobio.2013.06.006

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Gann, P. M., Milillo, M., Kwak, Y. I., Quintero, R., Waterman, P. E., & Lesho, E. (2013). Rapid and simultaneous detection of the chlorhexidine and mupirocin resistance genes qacA/B and mupA in clinical isolates of methicillin-resistant Staphylococcus aureus. Diagnostic Microbiology and Infectious Disease, 77(3), 270-272. https://doi.org/10.1016/j.diagmicrobio.2013.06.006

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title = "Rapid and simultaneous detection of the chlorhexidine and mupirocin resistance genes qacA/B and mupA in clinical isolates of methicillin-resistant Staphylococcus aureus",

abstract = "We describe a real-time PCR-based assay capable of simultaneously detecting femA (Staphylococcus aureus-specific), mecA (methicillin resistance), qacA/B (chlorhexidine tolerance), and mupA (high-level mupirocin resistance) from bacterial cells in less than 90 minutes. The assay was validated with 1968 clinical MRSA submitted to a surveillance network.",

keywords = "Chlorhexidine, MRSA, MupA, Mupirocin, QacA",

author = "Gann, {Patrick Mc} and Michael Milillo and Kwak, {Yoon I.} and Reyes Quintero and Waterman, {Paige E.} and Emil Lesho",

note = "Funding Information: Major funding for this study was provided by the US Army Medical Command and the Department of Defense Global Emerging Infections Surveillance and Response System. ",

year = "2013",

month = nov,

doi = "10.1016/j.diagmicrobio.2013.06.006",

language = "English",

volume = "77",

pages = "270--272",

journal = "Diagnostic Microbiology and Infectious Disease",

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number = "3",

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Gann, PM, Milillo, M, Kwak, YI, Quintero, R, Waterman, PE & Lesho, E 2013, 'Rapid and simultaneous detection of the chlorhexidine and mupirocin resistance genes qacA/B and mupA in clinical isolates of methicillin-resistant Staphylococcus aureus', Diagnostic Microbiology and Infectious Disease, vol. 77, no. 3, pp. 270-272. https://doi.org/10.1016/j.diagmicrobio.2013.06.006

Rapid and simultaneous detection of the chlorhexidine and mupirocin resistance genes qacA/B and mupA in clinical isolates of methicillin-resistant Staphylococcus aureus. / Gann, Patrick Mc; Milillo, Michael; Kwak, Yoon I. et al.
In: Diagnostic Microbiology and Infectious Disease, Vol. 77, No. 3, 11.2013, p. 270-272.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Rapid and simultaneous detection of the chlorhexidine and mupirocin resistance genes qacA/B and mupA in clinical isolates of methicillin-resistant Staphylococcus aureus

AU - Gann, Patrick Mc

AU - Milillo, Michael

AU - Kwak, Yoon I.

AU - Quintero, Reyes

AU - Waterman, Paige E.

AU - Lesho, Emil

N1 - Funding Information: Major funding for this study was provided by the US Army Medical Command and the Department of Defense Global Emerging Infections Surveillance and Response System.

PY - 2013/11

Y1 - 2013/11

N2 - We describe a real-time PCR-based assay capable of simultaneously detecting femA (Staphylococcus aureus-specific), mecA (methicillin resistance), qacA/B (chlorhexidine tolerance), and mupA (high-level mupirocin resistance) from bacterial cells in less than 90 minutes. The assay was validated with 1968 clinical MRSA submitted to a surveillance network.

AB - We describe a real-time PCR-based assay capable of simultaneously detecting femA (Staphylococcus aureus-specific), mecA (methicillin resistance), qacA/B (chlorhexidine tolerance), and mupA (high-level mupirocin resistance) from bacterial cells in less than 90 minutes. The assay was validated with 1968 clinical MRSA submitted to a surveillance network.

KW - Chlorhexidine

KW - MRSA

KW - MupA

KW - Mupirocin

KW - QacA

UR - http://www.scopus.com/inward/record.url?scp=84885370908&partnerID=8YFLogxK

U2 - 10.1016/j.diagmicrobio.2013.06.006

DO - 10.1016/j.diagmicrobio.2013.06.006

M3 - Article

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AN - SCOPUS:84885370908

SN - 0732-8893

VL - 77

SP - 270

EP - 272

JO - Diagnostic Microbiology and Infectious Disease

JF - Diagnostic Microbiology and Infectious Disease

IS - 3

ER -

Rapid and simultaneous detection of the chlorhexidine and mupirocin resistance genes qacA/B and mupA in clinical isolates of methicillin-resistant Staphylococcus aureus

Abstract

Keywords

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