Rapid purification and properties of protein kinase C from bovine adrenal medulla

Keith W. Brocklehurst; George Lee; Harvey B. Pollard

doi:10.1007/BF01116543

Rapid purification and properties of protein kinase C from bovine adrenal medulla

Keith W. Brocklehurst^*
, George Lee
, Harvey B. Pollard

^*Corresponding author for this work

Anatomy, Physiology, and Genetics

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Protein kinase C was purified from bovine adrenal medulla using a rapid procedure which resulted in an approximate 1500-fold increase in specific activity. The characteristics of the enzyme are reported and for the first time it is possible to compare the effects of TPA on secretion from intact and permeabilized cells with the effect of TPA on protein kinase C purified from the same secretory tissue.

Original language	English
Pages (from-to)	749-757
Number of pages	9
Journal	Bioscience Reports
Volume	6
Issue number	8
DOIs	https://doi.org/10.1007/BF01116543
State	Published - Aug 1986

Keywords

Adrenal medulla
protein kinase C

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10.1007/BF01116543

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title = "Rapid purification and properties of protein kinase C from bovine adrenal medulla",

abstract = "Protein kinase C was purified from bovine adrenal medulla using a rapid procedure which resulted in an approximate 1500-fold increase in specific activity. The characteristics of the enzyme are reported and for the first time it is possible to compare the effects of TPA on secretion from intact and permeabilized cells with the effect of TPA on protein kinase C purified from the same secretory tissue.",

keywords = "Adrenal medulla, protein kinase C",

author = "Brocklehurst, \{Keith W.\} and George Lee and Pollard, \{Harvey B.\}",

year = "1986",

month = aug,

doi = "10.1007/BF01116543",

language = "English",

volume = "6",

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journal = "Bioscience Reports",

issn = "0144-8463",

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T1 - Rapid purification and properties of protein kinase C from bovine adrenal medulla

AU - Brocklehurst, Keith W.

AU - Lee, George

AU - Pollard, Harvey B.

PY - 1986/8

Y1 - 1986/8

N2 - Protein kinase C was purified from bovine adrenal medulla using a rapid procedure which resulted in an approximate 1500-fold increase in specific activity. The characteristics of the enzyme are reported and for the first time it is possible to compare the effects of TPA on secretion from intact and permeabilized cells with the effect of TPA on protein kinase C purified from the same secretory tissue.

AB - Protein kinase C was purified from bovine adrenal medulla using a rapid procedure which resulted in an approximate 1500-fold increase in specific activity. The characteristics of the enzyme are reported and for the first time it is possible to compare the effects of TPA on secretion from intact and permeabilized cells with the effect of TPA on protein kinase C purified from the same secretory tissue.

KW - Adrenal medulla

KW - protein kinase C

UR - https://www.scopus.com/pages/publications/0022758268

U2 - 10.1007/BF01116543

DO - 10.1007/BF01116543

M3 - Article

C2 - 3814769

AN - SCOPUS:0022758268

SN - 0144-8463

VL - 6

SP - 749

EP - 757

JO - Bioscience Reports

JF - Bioscience Reports

IS - 8

ER -

Rapid purification and properties of protein kinase C from bovine adrenal medulla

Abstract

Keywords

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