Regulation of hepatic lipoprotein receptors during aging in the rat

D. Superfin; J. Thai; R. Sohn; S. Lear; M. B. Engler; D. L. Williams; M. M. Engler; S. K. Erickson

Regulation of hepatic lipoprotein receptors during aging in the rat

D. Superfin, J. Thai, R. Sohn, S. Lear, M. B. Engler, D. L. Williams, M. M. Engler, S. K. Erickson

Graduate School of Nursing

Research output: Contribution to journal › Article › peer-review

Abstract

Development of atherosclerosis correlates with increased plasma cholesterol (PC). PC levels increase in both humans and rats during aging. We hypothesized that alteration of hepatic lipoprotein (LP) receptors (R) during aging was responsible. Low density lipoprotein R (LDLR) and α2 macroglobulin R (LRP) remove apo E and apo B100 containing LP by receptor-mediated endocytosis while SR-B1 removes cholesterol esters from LP by selective uptake. Levels of mRNA for LDLR, LRP and SR-B1 were determined by Northern blot of total RNA isolated from young (15 wk), middle aged (15 mo), and aged (24 mo) male Fisher 344 rats (n=10 in each group). SR-B1 mRNA was increased ∼2 fold in aged livers compared to young (p<0.01). mRNA levels for LDLR and LRP showed no significant changes. Amounts of R proteins were estimated in liver membranes isolated from the same animals by Western blot. Preliminary results indicated LDLR decreased in the aged livers and SR-B1 increased. The data taken together suggest that age-related changes in expression of hepatic LP receptors are in part responsible for the PC changes.

Original language	English
Pages (from-to)	A686
Journal	FASEB Journal
Volume	12
Issue number	5
State	Published - 20 Mar 1998

Cite this

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title = "Regulation of hepatic lipoprotein receptors during aging in the rat",

abstract = "Development of atherosclerosis correlates with increased plasma cholesterol (PC). PC levels increase in both humans and rats during aging. We hypothesized that alteration of hepatic lipoprotein (LP) receptors (R) during aging was responsible. Low density lipoprotein R (LDLR) and α2 macroglobulin R (LRP) remove apo E and apo B100 containing LP by receptor-mediated endocytosis while SR-B1 removes cholesterol esters from LP by selective uptake. Levels of mRNA for LDLR, LRP and SR-B1 were determined by Northern blot of total RNA isolated from young (15 wk), middle aged (15 mo), and aged (24 mo) male Fisher 344 rats (n=10 in each group). SR-B1 mRNA was increased ∼2 fold in aged livers compared to young (p<0.01). mRNA levels for LDLR and LRP showed no significant changes. Amounts of R proteins were estimated in liver membranes isolated from the same animals by Western blot. Preliminary results indicated LDLR decreased in the aged livers and SR-B1 increased. The data taken together suggest that age-related changes in expression of hepatic LP receptors are in part responsible for the PC changes.",

author = "D. Superfin and J. Thai and R. Sohn and S. Lear and Engler, {M. B.} and Williams, {D. L.} and Engler, {M. M.} and Erickson, {S. K.}",

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TY - JOUR

T1 - Regulation of hepatic lipoprotein receptors during aging in the rat

AU - Superfin, D.

AU - Thai, J.

AU - Sohn, R.

AU - Lear, S.

AU - Engler, M. B.

AU - Williams, D. L.

AU - Engler, M. M.

AU - Erickson, S. K.

PY - 1998/3/20

Y1 - 1998/3/20

N2 - Development of atherosclerosis correlates with increased plasma cholesterol (PC). PC levels increase in both humans and rats during aging. We hypothesized that alteration of hepatic lipoprotein (LP) receptors (R) during aging was responsible. Low density lipoprotein R (LDLR) and α2 macroglobulin R (LRP) remove apo E and apo B100 containing LP by receptor-mediated endocytosis while SR-B1 removes cholesterol esters from LP by selective uptake. Levels of mRNA for LDLR, LRP and SR-B1 were determined by Northern blot of total RNA isolated from young (15 wk), middle aged (15 mo), and aged (24 mo) male Fisher 344 rats (n=10 in each group). SR-B1 mRNA was increased ∼2 fold in aged livers compared to young (p<0.01). mRNA levels for LDLR and LRP showed no significant changes. Amounts of R proteins were estimated in liver membranes isolated from the same animals by Western blot. Preliminary results indicated LDLR decreased in the aged livers and SR-B1 increased. The data taken together suggest that age-related changes in expression of hepatic LP receptors are in part responsible for the PC changes.

AB - Development of atherosclerosis correlates with increased plasma cholesterol (PC). PC levels increase in both humans and rats during aging. We hypothesized that alteration of hepatic lipoprotein (LP) receptors (R) during aging was responsible. Low density lipoprotein R (LDLR) and α2 macroglobulin R (LRP) remove apo E and apo B100 containing LP by receptor-mediated endocytosis while SR-B1 removes cholesterol esters from LP by selective uptake. Levels of mRNA for LDLR, LRP and SR-B1 were determined by Northern blot of total RNA isolated from young (15 wk), middle aged (15 mo), and aged (24 mo) male Fisher 344 rats (n=10 in each group). SR-B1 mRNA was increased ∼2 fold in aged livers compared to young (p<0.01). mRNA levels for LDLR and LRP showed no significant changes. Amounts of R proteins were estimated in liver membranes isolated from the same animals by Western blot. Preliminary results indicated LDLR decreased in the aged livers and SR-B1 increased. The data taken together suggest that age-related changes in expression of hepatic LP receptors are in part responsible for the PC changes.

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SN - 0892-6638

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