Regulation off constitutive nitric oxide synthase activity by the human heart

Brack G. Hattler*, Carmine V. Oddis, Adriana Zeevi, Hartmut Luss, Nish Shah, David A. Geller, Timothy R. Billiar, Richard L. Simmons, Mitchell S. Finkel

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

27 Scopus citations


We and others have provided indirect evidence for the presence of a constitutive nitric oxide synthase (cNOS) in the mammalian heart. We now provide more direct evidence for the regulation of a myocardial cNOS in the hearts of patients undergoing elective cardiopulmonary bypass (CPB). cNOS enzyme activity was demonstrable in both cytosolic (8.3 ± 0.02 pmol/min/mg) and membrane (11.1 ± 0.4 pmol/min/mg) preparations derived from human atrial pectinate muscles obtained at the rime of CPB (n = 6). Plasma nitrite (NO2-) + plasma nitrate (NO3-) levels from the beating hearts of patients before bypass were reduced from 146 ± 33 to 5.1 ± 50 pmol/min/g after cardiac arrest during CPB (n = 23; p < 0.002 by Student's t test). Thus, the human myocardium constitutively produces nitric oxide that is regulated by the contractile state of the heart.

Original languageEnglish
Pages (from-to)957-959
Number of pages3
JournalThe American Journal of Cardiology
Issue number12
StatePublished - 1 Nov 1995


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