Regulation off constitutive nitric oxide synthase activity by the human heart

Brack G. Hattler; Carmine V. Oddis; Adriana Zeevi; Hartmut Luss; Nish Shah; David A. Geller; Timothy R. Billiar; Richard L. Simmons; Mitchell S. Finkel

doi:10.1016/S0002-9149(99)80269-0

Regulation off constitutive nitric oxide synthase activity by the human heart

Brack G. Hattler^*, Carmine V. Oddis, Adriana Zeevi, Hartmut Luss, Nish Shah, David A. Geller, Timothy R. Billiar, Richard L. Simmons, Mitchell S. Finkel

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

We and others have provided indirect evidence for the presence of a constitutive nitric oxide synthase (cNOS) in the mammalian heart. We now provide more direct evidence for the regulation of a myocardial cNOS in the hearts of patients undergoing elective cardiopulmonary bypass (CPB). cNOS enzyme activity was demonstrable in both cytosolic (8.3 ± 0.02 pmol/min/mg) and membrane (11.1 ± 0.4 pmol/min/mg) preparations derived from human atrial pectinate muscles obtained at the rime of CPB (n = 6). Plasma nitrite (NO₂^-) + plasma nitrate (NO₃^-) levels from the beating hearts of patients before bypass were reduced from 146 ± 33 to 5.1 ± 50 pmol/min/g after cardiac arrest during CPB (n = 23; p < 0.002 by Student's t test). Thus, the human myocardium constitutively produces nitric oxide that is regulated by the contractile state of the heart.

Original language	English
Pages (from-to)	957-959
Number of pages	3
Journal	The American Journal of Cardiology
Volume	76
Issue number	12
DOIs	https://doi.org/10.1016/S0002-9149(99)80269-0
State	Published - 1 Nov 1995
Externally published	Yes

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title = "Regulation off constitutive nitric oxide synthase activity by the human heart",

abstract = "We and others have provided indirect evidence for the presence of a constitutive nitric oxide synthase (cNOS) in the mammalian heart. We now provide more direct evidence for the regulation of a myocardial cNOS in the hearts of patients undergoing elective cardiopulmonary bypass (CPB). cNOS enzyme activity was demonstrable in both cytosolic (8.3 ± 0.02 pmol/min/mg) and membrane (11.1 ± 0.4 pmol/min/mg) preparations derived from human atrial pectinate muscles obtained at the rime of CPB (n = 6). Plasma nitrite (NO2-) + plasma nitrate (NO3-) levels from the beating hearts of patients before bypass were reduced from 146 ± 33 to 5.1 ± 50 pmol/min/g after cardiac arrest during CPB (n = 23; p < 0.002 by Student's t test). Thus, the human myocardium constitutively produces nitric oxide that is regulated by the contractile state of the heart.",

author = "Hattler, {Brack G.} and Oddis, {Carmine V.} and Adriana Zeevi and Hartmut Luss and Nish Shah and Geller, {David A.} and Billiar, {Timothy R.} and Simmons, {Richard L.} and Finkel, {Mitchell S.}",

note = "Funding Information: From the Departments of Surge Medicine, Pharmacology, and Patholoav. Untversitv of Pjttsbura xr School of Medicine. Ptttsburah. Pennsyl&a. This study was supported by National Institutes of Her& Grants HL53372 and GM377.53. Manuscript recerved June 22, 1995; revised manuscript received and accepted July 27, 1995.",

year = "1995",

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doi = "10.1016/S0002-9149(99)80269-0",

language = "English",

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T1 - Regulation off constitutive nitric oxide synthase activity by the human heart

AU - Hattler, Brack G.

AU - Oddis, Carmine V.

AU - Zeevi, Adriana

AU - Luss, Hartmut

AU - Shah, Nish

AU - Geller, David A.

AU - Billiar, Timothy R.

AU - Simmons, Richard L.

AU - Finkel, Mitchell S.

N1 - Funding Information: From the Departments of Surge Medicine, Pharmacology, and Patholoav. Untversitv of Pjttsbura xr School of Medicine. Ptttsburah. Pennsyl&a. This study was supported by National Institutes of Her& Grants HL53372 and GM377.53. Manuscript recerved June 22, 1995; revised manuscript received and accepted July 27, 1995.

PY - 1995/11/1

Y1 - 1995/11/1

N2 - We and others have provided indirect evidence for the presence of a constitutive nitric oxide synthase (cNOS) in the mammalian heart. We now provide more direct evidence for the regulation of a myocardial cNOS in the hearts of patients undergoing elective cardiopulmonary bypass (CPB). cNOS enzyme activity was demonstrable in both cytosolic (8.3 ± 0.02 pmol/min/mg) and membrane (11.1 ± 0.4 pmol/min/mg) preparations derived from human atrial pectinate muscles obtained at the rime of CPB (n = 6). Plasma nitrite (NO2-) + plasma nitrate (NO3-) levels from the beating hearts of patients before bypass were reduced from 146 ± 33 to 5.1 ± 50 pmol/min/g after cardiac arrest during CPB (n = 23; p < 0.002 by Student's t test). Thus, the human myocardium constitutively produces nitric oxide that is regulated by the contractile state of the heart.

AB - We and others have provided indirect evidence for the presence of a constitutive nitric oxide synthase (cNOS) in the mammalian heart. We now provide more direct evidence for the regulation of a myocardial cNOS in the hearts of patients undergoing elective cardiopulmonary bypass (CPB). cNOS enzyme activity was demonstrable in both cytosolic (8.3 ± 0.02 pmol/min/mg) and membrane (11.1 ± 0.4 pmol/min/mg) preparations derived from human atrial pectinate muscles obtained at the rime of CPB (n = 6). Plasma nitrite (NO2-) + plasma nitrate (NO3-) levels from the beating hearts of patients before bypass were reduced from 146 ± 33 to 5.1 ± 50 pmol/min/g after cardiac arrest during CPB (n = 23; p < 0.002 by Student's t test). Thus, the human myocardium constitutively produces nitric oxide that is regulated by the contractile state of the heart.

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JO - The American Journal of Cardiology

JF - The American Journal of Cardiology

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Regulation off constitutive nitric oxide synthase activity by the human heart

Abstract

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