Responses to NMDA in cultured hippocampal neurons from trisomy 16 embryonic mice

R. J. Siarey; E. J. Coan; S. I. Rapoport; Z. Galdzicki

doi:10.1016/S0304-3940(97)00581-8

Responses to NMDA in cultured hippocampal neurons from trisomy 16 embryonic mice

R. J. Siarey
, E. J. Coan
, S. I. Rapoport
, Z. Galdzicki^*

^*Corresponding author for this work

Anatomy, Physiology, and Genetics

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

The trisomy 16 (Ts16) mouse is regarded as a model of human trisomy 21 (Ts21), or Down syndrome. The ionic current evoked by the glutamate receptor agonist N-methyl-D-aspartate (NMDA) was investigated in cultured hippocampal neurons from embryonic Ts16 and control diploid mice. In both Ts16 and control neurons, NMDA- (6-150 μM) evoked a similar inward current. The reversal potential, the minimum current, the dose response plot of the conductance, the effect of Mg²⁺ on the current-voltage plot and the inhibition by D-2-amino-5-phosphonovaleric acid (AP5; 50 μM) showed no significant difference between Ts16 and control neurons. These data suggest that, although voltage-dependent ion channels are reported to have altered active properties to Ts16 neurons, NMDA-evoked currents are not altered.

Original language	English
Pages (from-to)	131-134
Number of pages	4
Journal	Neuroscience Letters
Volume	232
Issue number	3
DOIs	https://doi.org/10.1016/S0304-3940(97)00581-8
State	Published - 5 Sep 1997

Keywords

Down syndrome
Glutamate
Hippocampus
N-Methyl-D-aspartate
Trisomy 16
Whole-cell patch-clamp

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10.1016/S0304-3940(97)00581-8

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title = "Responses to NMDA in cultured hippocampal neurons from trisomy 16 embryonic mice",

abstract = "The trisomy 16 (Ts16) mouse is regarded as a model of human trisomy 21 (Ts21), or Down syndrome. The ionic current evoked by the glutamate receptor agonist N-methyl-D-aspartate (NMDA) was investigated in cultured hippocampal neurons from embryonic Ts16 and control diploid mice. In both Ts16 and control neurons, NMDA- (6-150 μM) evoked a similar inward current. The reversal potential, the minimum current, the dose response plot of the conductance, the effect of Mg2+ on the current-voltage plot and the inhibition by D-2-amino-5-phosphonovaleric acid (AP5; 50 μM) showed no significant difference between Ts16 and control neurons. These data suggest that, although voltage-dependent ion channels are reported to have altered active properties to Ts16 neurons, NMDA-evoked currents are not altered.",

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AU - Siarey, R. J.

AU - Coan, E. J.

AU - Rapoport, S. I.

AU - Galdzicki, Z.

PY - 1997/9/5

Y1 - 1997/9/5

N2 - The trisomy 16 (Ts16) mouse is regarded as a model of human trisomy 21 (Ts21), or Down syndrome. The ionic current evoked by the glutamate receptor agonist N-methyl-D-aspartate (NMDA) was investigated in cultured hippocampal neurons from embryonic Ts16 and control diploid mice. In both Ts16 and control neurons, NMDA- (6-150 μM) evoked a similar inward current. The reversal potential, the minimum current, the dose response plot of the conductance, the effect of Mg2+ on the current-voltage plot and the inhibition by D-2-amino-5-phosphonovaleric acid (AP5; 50 μM) showed no significant difference between Ts16 and control neurons. These data suggest that, although voltage-dependent ion channels are reported to have altered active properties to Ts16 neurons, NMDA-evoked currents are not altered.

AB - The trisomy 16 (Ts16) mouse is regarded as a model of human trisomy 21 (Ts21), or Down syndrome. The ionic current evoked by the glutamate receptor agonist N-methyl-D-aspartate (NMDA) was investigated in cultured hippocampal neurons from embryonic Ts16 and control diploid mice. In both Ts16 and control neurons, NMDA- (6-150 μM) evoked a similar inward current. The reversal potential, the minimum current, the dose response plot of the conductance, the effect of Mg2+ on the current-voltage plot and the inhibition by D-2-amino-5-phosphonovaleric acid (AP5; 50 μM) showed no significant difference between Ts16 and control neurons. These data suggest that, although voltage-dependent ion channels are reported to have altered active properties to Ts16 neurons, NMDA-evoked currents are not altered.

KW - Down syndrome

KW - Glutamate

KW - Hippocampus

KW - N-Methyl-D-aspartate

KW - Trisomy 16

KW - Whole-cell patch-clamp

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JF - Neuroscience Letters

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ER -

Responses to NMDA in cultured hippocampal neurons from trisomy 16 embryonic mice

Abstract

Keywords

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