Restoration of HIV-specific cell-mediated immune responses by interleukin-12 in vitro

Mario Clerici, Daniel R. Lucey, Jay A. Berzofsky, Ligia A. Pinto, Thomas A. Wynn, Stephen P. Blatt, Matthew J. Dolan, Craig W. Hendrix, Stanley F. Wolf, Gene M. Shearer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

389 Scopus citations


Peripheral blood mononuclear cells (PBMCs) from many asymptomatic individuals infected with human immunodeficiency virus-type 1 (HIV) are unresponsive as measured by in vitro T cell proliferation and interleukin-2 (IL-2) production to influenza virus and synthetic peptides of HIV envelope (Env). Strong influenza virus- and Env-stimulated IL-2 responses and T cell proliferation were restored when cultures were stimulated in the presence of IL-12. Interferon-γ production by PBMCs from HIV seropositive (HIV +) patients was also restored with IL-12. Furthermore, in vitro antigen-specific production of IL-2 and proliferation of PBMCs from HIV - donors were suppressed by antibody to IL-12, but were not enhanced by addition of exogenous IL-12. Thus, IL12 may be limiting in PBMCs from HIV+ but not HIV- individuals. These findings demonstrate that IL-12 can restore HIV-specific cell-mediated immunity in vitro in HIV-infected individuals and suggest a potential use of IL-12 in augmenting the diminished immunologic functions associated with HIV infection.

Original languageEnglish
Pages (from-to)1721-1724
Number of pages4
Issue number5140
StatePublished - 1993
Externally publishedYes


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