TY - JOUR
T1 - Restricted replication and lysosomal trafficking of yellow fever 17D vaccine virus in human dendritic cells
AU - Palmer, Dupeh R.
AU - Fernandez, Stefan
AU - Bisbing, John
AU - Peachman, Kristina K.
AU - Rao, Mangala
AU - Barvir, Dave
AU - Gunther, Vicky
AU - Burgess, Timothy
AU - Kohno, Yukari
AU - Padmanabhan, R.
AU - Sun, Wellington
PY - 2007/1
Y1 - 2007/1
N2 - The yellow fever virus attenuated 17D vaccine strain is a safe and effective vaccine and a valuable model system for evaluating immune responses against attenuated viral variants. This study compared the in vitro interactions of the commercially available yellow fever vaccine (YF-VAX), Dengue virus and the live-attenuated dengue vaccine PDK50 with dendritic cells (DCs), the main antigen-presenting cells at the initiation of immune responses. Similar to PDK50, infection with YF-VAX generated activated DCs; however, for YF-VAX, activation occurred with limited intracellular virus replication. The majority of internalized virus co-localized with endolysosomal markers within 90 min, suggesting that YF-VAX is processed rapidly in DCs. These results indicate that restricted virus replication and lysosomal compartmentalization may be important contributing factors to the success of the YF-VAX vaccine.
AB - The yellow fever virus attenuated 17D vaccine strain is a safe and effective vaccine and a valuable model system for evaluating immune responses against attenuated viral variants. This study compared the in vitro interactions of the commercially available yellow fever vaccine (YF-VAX), Dengue virus and the live-attenuated dengue vaccine PDK50 with dendritic cells (DCs), the main antigen-presenting cells at the initiation of immune responses. Similar to PDK50, infection with YF-VAX generated activated DCs; however, for YF-VAX, activation occurred with limited intracellular virus replication. The majority of internalized virus co-localized with endolysosomal markers within 90 min, suggesting that YF-VAX is processed rapidly in DCs. These results indicate that restricted virus replication and lysosomal compartmentalization may be important contributing factors to the success of the YF-VAX vaccine.
UR - http://www.scopus.com/inward/record.url?scp=33846130030&partnerID=8YFLogxK
U2 - 10.1099/vir.0.82272-0
DO - 10.1099/vir.0.82272-0
M3 - Article
C2 - 17170447
AN - SCOPUS:33846130030
SN - 0022-1317
VL - 88
SP - 148
EP - 156
JO - Journal of General Virology
JF - Journal of General Virology
IS - 1
ER -