Restricted replication and lysosomal trafficking of yellow fever 17D vaccine virus in human dendritic cells

Dupeh R. Palmer*, Stefan Fernandez, John Bisbing, Kristina K. Peachman, Mangala Rao, Dave Barvir, Vicky Gunther, Timothy Burgess, Yukari Kohno, R. Padmanabhan, Wellington Sun

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

32 Scopus citations

Abstract

The yellow fever virus attenuated 17D vaccine strain is a safe and effective vaccine and a valuable model system for evaluating immune responses against attenuated viral variants. This study compared the in vitro interactions of the commercially available yellow fever vaccine (YF-VAX), Dengue virus and the live-attenuated dengue vaccine PDK50 with dendritic cells (DCs), the main antigen-presenting cells at the initiation of immune responses. Similar to PDK50, infection with YF-VAX generated activated DCs; however, for YF-VAX, activation occurred with limited intracellular virus replication. The majority of internalized virus co-localized with endolysosomal markers within 90 min, suggesting that YF-VAX is processed rapidly in DCs. These results indicate that restricted virus replication and lysosomal compartmentalization may be important contributing factors to the success of the YF-VAX vaccine.

Original languageEnglish
Pages (from-to)148-156
Number of pages9
JournalJournal of General Virology
Volume88
Issue number1
DOIs
StatePublished - Jan 2007

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