Restricted replication and lysosomal trafficking of yellow fever 17D vaccine virus in human dendritic cells

Dupeh R. Palmer; Stefan Fernandez; John Bisbing; Kristina K. Peachman; Mangala Rao; Dave Barvir; Vicky Gunther; Timothy Burgess; Yukari Kohno; R. Padmanabhan; Wellington Sun

doi:10.1099/vir.0.82272-0

Restricted replication and lysosomal trafficking of yellow fever 17D vaccine virus in human dendritic cells

Dupeh R. Palmer^*
, Stefan Fernandez
, John Bisbing
, Kristina K. Peachman
, Mangala Rao
, Dave Barvir
, Vicky Gunther
, Timothy Burgess
, Yukari Kohno
, R. Padmanabhan
, Wellington Sun

^*Corresponding author for this work

Infectious Disease Clinical Research Program (IDCRP)

Research output: Contribution to journal › Article › peer-review

34 Scopus citations

Abstract

The yellow fever virus attenuated 17D vaccine strain is a safe and effective vaccine and a valuable model system for evaluating immune responses against attenuated viral variants. This study compared the in vitro interactions of the commercially available yellow fever vaccine (YF-VAX), Dengue virus and the live-attenuated dengue vaccine PDK50 with dendritic cells (DCs), the main antigen-presenting cells at the initiation of immune responses. Similar to PDK50, infection with YF-VAX generated activated DCs; however, for YF-VAX, activation occurred with limited intracellular virus replication. The majority of internalized virus co-localized with endolysosomal markers within 90 min, suggesting that YF-VAX is processed rapidly in DCs. These results indicate that restricted virus replication and lysosomal compartmentalization may be important contributing factors to the success of the YF-VAX vaccine.

Original language	English
Pages (from-to)	148-156
Number of pages	9
Journal	Journal of General Virology
Volume	88
Issue number	1
DOIs	https://doi.org/10.1099/vir.0.82272-0
State	Published - Jan 2007

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title = "Restricted replication and lysosomal trafficking of yellow fever 17D vaccine virus in human dendritic cells",

abstract = "The yellow fever virus attenuated 17D vaccine strain is a safe and effective vaccine and a valuable model system for evaluating immune responses against attenuated viral variants. This study compared the in vitro interactions of the commercially available yellow fever vaccine (YF-VAX), Dengue virus and the live-attenuated dengue vaccine PDK50 with dendritic cells (DCs), the main antigen-presenting cells at the initiation of immune responses. Similar to PDK50, infection with YF-VAX generated activated DCs; however, for YF-VAX, activation occurred with limited intracellular virus replication. The majority of internalized virus co-localized with endolysosomal markers within 90 min, suggesting that YF-VAX is processed rapidly in DCs. These results indicate that restricted virus replication and lysosomal compartmentalization may be important contributing factors to the success of the YF-VAX vaccine.",

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AU - Palmer, Dupeh R.

AU - Fernandez, Stefan

AU - Bisbing, John

AU - Peachman, Kristina K.

AU - Rao, Mangala

AU - Barvir, Dave

AU - Gunther, Vicky

AU - Burgess, Timothy

AU - Kohno, Yukari

AU - Padmanabhan, R.

AU - Sun, Wellington

PY - 2007/1

Y1 - 2007/1

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AB - The yellow fever virus attenuated 17D vaccine strain is a safe and effective vaccine and a valuable model system for evaluating immune responses against attenuated viral variants. This study compared the in vitro interactions of the commercially available yellow fever vaccine (YF-VAX), Dengue virus and the live-attenuated dengue vaccine PDK50 with dendritic cells (DCs), the main antigen-presenting cells at the initiation of immune responses. Similar to PDK50, infection with YF-VAX generated activated DCs; however, for YF-VAX, activation occurred with limited intracellular virus replication. The majority of internalized virus co-localized with endolysosomal markers within 90 min, suggesting that YF-VAX is processed rapidly in DCs. These results indicate that restricted virus replication and lysosomal compartmentalization may be important contributing factors to the success of the YF-VAX vaccine.

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AN - SCOPUS:33846130030

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SP - 148

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JO - Journal of General Virology

JF - Journal of General Virology

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Restricted replication and lysosomal trafficking of yellow fever 17D vaccine virus in human dendritic cells

Abstract

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