TY - JOUR
T1 - Restriction endonuclease fingerprinting of herpes simplex virus DNA
T2 - A novel epidemiological tool applied to a nosocomial outbreak
AU - Buchman, Timothy G.
AU - Roizman, Bernard
AU - Adams, Garrett
AU - Stover, Beth Hewitt
AU - Buchman, Timothy G.
AU - Roizman, Bernard
AU - Adams, Garrett
AU - Stover, Beth Hewitt
N1 - Funding Information:
Received for publication December 19, 1977, and in revised form May 4, 1978. The studies conductedat the University of Chicago were aided by grant no. VC 103L from the American Cancer Society, grant no. CA 08494 from the National Cancer Institute, and grant no. CA 19264 from the National Institutes of Health. Dr. Buchman is the recipient of U.S. Public Health Service training grant no. 5 T32 GMO 7281 through the Medical Scientist Training Program. Please address requests for reprints to Dr. Bernard Roiz man, Kovler Viral Oncology Laboratories, Division of Biological Sciences, University of Chicago, 910 East 58th Street, Chicago, Illinois60637.
PY - 1978/10
Y1 - 1978/10
N2 - In a blind study, 14 isolates of herpes simplex virus type 1 (HSV-1) that included nine isolates from a temporal cluster of HSV infections in a hospital Pediatric Intensive Care Unit and five unrelated isolates were analyzed by digestion of their DNA with four restriction endonucleases. These enzymes (Hsu1, Bgl11, EcoRl, and Hpal) cleave the DNA in about 52 sites. To date, at least 16 sites have been found to be variable in the sense that they may be present or absent independently of any other cleavage site. This characteristic is stable, and no change was observed on serial propagation of the strains in culture or following repeated isolation, as long as 12 years apart, from humans. Analyses of the isolates readily discriminated between those belonging to the temporal cluster of hospital infections and the unrelated strains. They also showed that there were two independent introductions of HSV-1 into the Pediatric Intensive Care Unit resulting in two clusters of epidemiologically related infections. This type of analysis has the potential of becoming a powerful tool for tracing the spread of HSV-1 and very likely of other herpesviruses in the human population.
AB - In a blind study, 14 isolates of herpes simplex virus type 1 (HSV-1) that included nine isolates from a temporal cluster of HSV infections in a hospital Pediatric Intensive Care Unit and five unrelated isolates were analyzed by digestion of their DNA with four restriction endonucleases. These enzymes (Hsu1, Bgl11, EcoRl, and Hpal) cleave the DNA in about 52 sites. To date, at least 16 sites have been found to be variable in the sense that they may be present or absent independently of any other cleavage site. This characteristic is stable, and no change was observed on serial propagation of the strains in culture or following repeated isolation, as long as 12 years apart, from humans. Analyses of the isolates readily discriminated between those belonging to the temporal cluster of hospital infections and the unrelated strains. They also showed that there were two independent introductions of HSV-1 into the Pediatric Intensive Care Unit resulting in two clusters of epidemiologically related infections. This type of analysis has the potential of becoming a powerful tool for tracing the spread of HSV-1 and very likely of other herpesviruses in the human population.
UR - http://www.scopus.com/inward/record.url?scp=0018166573&partnerID=8YFLogxK
U2 - 10.1093/infdis/138.4.488
DO - 10.1093/infdis/138.4.488
M3 - Article
C2 - 213496
AN - SCOPUS:0018166573
SN - 0022-1899
VL - 138
SP - 488
EP - 498
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -