Role of c-myc and CD45 in spontaneous and anti-receptor-induced apoptosis in adult murine B cells

David W. Scott, Marinus Lamers, Georges Köhler, Charles L. Sidman, Bourke Maddox, Rita Carsetti

Research output: Contribution to journalArticlepeer-review

15 Scopus citations


Although adult murine B cells can be stimulated to proliferate by IgM receptor cross-linking, we and others have shown that these cells will undergo apoptosis in vitro in a dose-, time- and temperature-dependent manner with polyclonal but not monoclonal anti-IgM. To test the role of c-myc and cell cycle progression in B cell apoptosis, we examined normal, Sp6 anti-TNP Ig and E(μ)-myc transgenic splenocytes for receptor-mediated apoptosis in vitro. In normal mice, both spontaneous and anti-IgM-induced programmed cell death were specifically blocked by antisense oligodeoxynucleotides for the c-myc proto-oncogene, whereas nonsense myc oligonucleotides and irrelevant oligonucleotides had only a minor effect. Similarly, TNP-dextran-induced apoptosis in Sp6 anti-TNP transgenics was inhibited by antisense c-myc. This effect was not due to the mitogenic effects of unmethylated CpG-containing sequences because ones lacking this motif, as well as methylated oligonucleotides containing this motif, prevented apoptosis, and mitogenic doses of lipopolysaccharide failed to inhibit anti-IgM-driven cell death. Importantly, antisense c-myc also prevented the anti-IgM-induced increase in myc protein species. Moreover, spontaneous apoptosis in vitro was exaggerated in E(μ)-myc transgenic a cells. To examine the role of CD45 in anti-IgM-induced apoptosis, we treated spleen cells from CD45 knockout mice, which do not proliferate with anti-IgM, and found that B cells from these underwent apoptosis normally despite the lack of entry into S. These data suggest that anti-IgM driven apoptosis does not require CD45. Rather, apoptosis may be due to an overexpression of myc protein in the absence of signals which can drive B cells productively into S, but the failure to proliferate normally is insufficient for apoptosis to occur.

Original languageEnglish
Pages (from-to)1375-1385
Number of pages11
JournalInternational Immunology
Issue number9
StatePublished - 1996
Externally publishedYes


  • Antisense
  • Apoptosis B cell
  • IgM
  • Oncogene


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