Role of inducible nitric oxide synthase in postoperative intestinal smooth muscle dysfunction in rodents

Jörg C. Kalff, Wolfgang H. Schraut, Timothy R. Billiar, Richard L. Simmons, Anthony J. Bauer*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

248 Scopus citations


Background and Aims: We have shown that intestinal manipulation leads to a significant inhibition of circular muscle contraction. We hypothesized that the inflammatory mediator inducible nitric oxide (NO) plays a role in surgically induced ileus. Methods: Rats and inducible NO synthase (iNOS) knockout and wild-type mice underwent a simple intestinal manipulation. Reverse-transcription polymerase chain reaction and immunohistochemistry were used to detect and localize iNOS expression. Nitrite and NO production were measured in muscularis cultures. Spontaneous and bethanechol-stimulated jejunal circular muscle contractions were measured in an organ bath. Results: Intestinal manipulation resulted in significant iNOS messenger RNA induction in mucosa and muscularis. Immunohistochemistry localized iNOS in phagocytes within the muscularis. Nitrite and NO production increased 59.8-fold 24 hours after manipulation. L-n6-(1-iminoethyl) lysine (L-NIL) inhibited this response. In control rats, selective iNOS inhibition did not increase spontaneous muscle activity, but after manipulation L-NIL significantly improved spontaneous activity, iNOS knockout mice showed a significant 81% decrease in neutrophil infiltration into the muscularis after intestinal manipulation compared with wild-types. Contractile activity was normal in knockout mice after intestinal manipulation. Conclusions: These results show that leukocyte-derived inducible NO inhibits gastrointestinal motility after manipulation and plays an essential role in the initiation of intestinal inflammation.

Original languageEnglish
Pages (from-to)316-327
Number of pages12
Issue number2
StatePublished - 2000
Externally publishedYes


Dive into the research topics of 'Role of inducible nitric oxide synthase in postoperative intestinal smooth muscle dysfunction in rodents'. Together they form a unique fingerprint.

Cite this