TY - JOUR
T1 - Selective vulnerability of the cerebral vasculature to blast injury in a rat model of mild traumatic brain injury
AU - Gama Sosa, Miguel A.
AU - De Gasperi, Rita
AU - Janssen, Pierce L.
AU - Yuk, Frank J.
AU - Anazodo, Pamela C.
AU - Pricop, Paul E.
AU - Paulino, Alejandro J.
AU - Wicinski, Bridget
AU - Shaughness, Michael C.
AU - Maudlin-Jeronimo, Eric
AU - Hall, Aaron A.
AU - Dickstein, Dara L.
AU - McCarron, Richard M.
AU - Chavko, Mikulas
AU - Hof, Patrick R.
AU - Ahlers, Stephen T.
AU - Elder, Gregory A.
N1 - Publisher Copyright:
© 2014 Gama Sosa et al.; licensee BioMed Central Ltd.
PY - 2014/1/27
Y1 - 2014/1/27
N2 - Background: Blast-related traumatic brain injury (TBI) is a common cause of injury in the military operations in Iraq and Afghanistan. How the primary blast wave affects the brain is not well understood. The aim of the present study was to examine whether blast exposure affects the cerebral vasculature in a rodent model. We analyzed the brains of rats exposed to single or multiple (three) 74.5 kPa blast exposures, conditions that mimic a mild TBI. Rats were sacrificed 24 hours or between 6 and 10 months after exposure. Blast-induced cerebral vascular pathology was examined by a combination of light microscopy, immunohistochemistry, and electron microscopy. Results: We describe a selective vascular pathology that is present acutely at 24 hours after injury. The vascular pathology is found at the margins of focal shear-related injuries that, as we previously showed, typically follow the patterns of penetrating cortical vessels. However, changes in the microvasculature extend beyond the margins of such lesions. Electron microscopy revealed that microvascular pathology is found in regions of the brain with an otherwise normal neuropil. This initial injury leads to chronic changes in the microvasculature that are still evident many months after the initial blast exposure. Conclusions: These studies suggest that vascular pathology may be a central mechanism in the induction of chronic blast-related injury.
AB - Background: Blast-related traumatic brain injury (TBI) is a common cause of injury in the military operations in Iraq and Afghanistan. How the primary blast wave affects the brain is not well understood. The aim of the present study was to examine whether blast exposure affects the cerebral vasculature in a rodent model. We analyzed the brains of rats exposed to single or multiple (three) 74.5 kPa blast exposures, conditions that mimic a mild TBI. Rats were sacrificed 24 hours or between 6 and 10 months after exposure. Blast-induced cerebral vascular pathology was examined by a combination of light microscopy, immunohistochemistry, and electron microscopy. Results: We describe a selective vascular pathology that is present acutely at 24 hours after injury. The vascular pathology is found at the margins of focal shear-related injuries that, as we previously showed, typically follow the patterns of penetrating cortical vessels. However, changes in the microvasculature extend beyond the margins of such lesions. Electron microscopy revealed that microvascular pathology is found in regions of the brain with an otherwise normal neuropil. This initial injury leads to chronic changes in the microvasculature that are still evident many months after the initial blast exposure. Conclusions: These studies suggest that vascular pathology may be a central mechanism in the induction of chronic blast-related injury.
KW - Blast
KW - Rat
KW - Traumatic brain injury
KW - Vascular pathology
UR - http://www.scopus.com/inward/record.url?scp=84920756803&partnerID=8YFLogxK
U2 - 10.1186/2051-5960-2-67
DO - 10.1186/2051-5960-2-67
M3 - Article
C2 - 24938728
AN - SCOPUS:84920756803
SN - 2051-5960
VL - 2
JO - Acta Neuropathologica Communications
JF - Acta Neuropathologica Communications
IS - 1
M1 - 67
ER -