TY - JOUR
T1 - Short communication
T2 - HIV type 1 genetic diversity among tea plantation workers in Kericho, Kenya
AU - Arroyo, Miguel A.
AU - Sateren, Warren B.
AU - Foglia, Ginamarie
AU - Kibaya, Rukia
AU - Langat, Lilian
AU - Wasunna, Monique
AU - Bautista, Christian T.
AU - Scott, Paul T.
AU - Shaffer, Douglas N.
AU - Robb, Merlin L.
AU - Michael, Nelson L.
AU - Birx, Deborah L.
AU - McCutchan, Francine E.
PY - 2009/11/1
Y1 - 2009/11/1
N2 - In preparation for HIV-1 vaccine trials in Kenya, 2801 study volunteers, from a tea plantation in Kericho, were recruited as part of a prospective vaccine cohort development study. Cryopreserved plasma was available from 401 HIV-positive volunteers, and was the source of viral RNA for genotyping by the multiregion hybridization assay (MHA). Logistic regression was performed to determine association of risk factors and HIV-1 recombinant and dual infections. At baseline, HIV-1 subtype A was the dominant circulating pure subtype (56%), followed by subtype D (10%) and C (5%). Recombinant HIV-1 strains accounted for almost one-third of all infections (29%), with 7% infected with a dual strain of the HIV-1 variants described. A higher number of HIV-1 recombinant and dual infections was observed among volunteers who were 18-24 and 25-29 years of age, affiliated with the Luo tribe, had been married two or more times, reported not being circumcised, and had STI symptoms in the past 6 months. Adjusted odds ratios (AOR) significantly associated with HIV-1 recombinant and dual infection were age difference from current spouse (5-9 years; AOR=2.5, 95% CI=1.2-5.3 and ≥10 years; AOR=3.1, 95% CI=1.5-6.4) and reported STI symptoms in the past 6 months (AOR=4.8, 95% CI=2.0-11.6), respectively. In conclusion, our results suggest that there is considerable heterogeneity with respect to HIV-1 subtype diversity in this population that should be considered in the planning for future vaccine trials in the region.
AB - In preparation for HIV-1 vaccine trials in Kenya, 2801 study volunteers, from a tea plantation in Kericho, were recruited as part of a prospective vaccine cohort development study. Cryopreserved plasma was available from 401 HIV-positive volunteers, and was the source of viral RNA for genotyping by the multiregion hybridization assay (MHA). Logistic regression was performed to determine association of risk factors and HIV-1 recombinant and dual infections. At baseline, HIV-1 subtype A was the dominant circulating pure subtype (56%), followed by subtype D (10%) and C (5%). Recombinant HIV-1 strains accounted for almost one-third of all infections (29%), with 7% infected with a dual strain of the HIV-1 variants described. A higher number of HIV-1 recombinant and dual infections was observed among volunteers who were 18-24 and 25-29 years of age, affiliated with the Luo tribe, had been married two or more times, reported not being circumcised, and had STI symptoms in the past 6 months. Adjusted odds ratios (AOR) significantly associated with HIV-1 recombinant and dual infection were age difference from current spouse (5-9 years; AOR=2.5, 95% CI=1.2-5.3 and ≥10 years; AOR=3.1, 95% CI=1.5-6.4) and reported STI symptoms in the past 6 months (AOR=4.8, 95% CI=2.0-11.6), respectively. In conclusion, our results suggest that there is considerable heterogeneity with respect to HIV-1 subtype diversity in this population that should be considered in the planning for future vaccine trials in the region.
UR - http://www.scopus.com/inward/record.url?scp=71949091166&partnerID=8YFLogxK
U2 - 10.1089/aid.2009.0092
DO - 10.1089/aid.2009.0092
M3 - Article
C2 - 19943788
AN - SCOPUS:71949091166
SN - 0889-2229
VL - 25
SP - 1061
EP - 1064
JO - AIDS Research and Human Retroviruses
JF - AIDS Research and Human Retroviruses
IS - 11
ER -