Short report: Enumerating leptospires using the Coulter counter

Christina M. Humberd*, Clinton K. Murray, Saundra K. Stuart, Barbara A. Reeb, Duane R. Hospenthal

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

The currently accepted gold standard for enumeration of leptospires is tabulation of organisms using a Petroff-Hausser counting chamber and dark-field microscopy, a technically demanding, time-consuming technique. Quantification of leptospires with a Coulter counter produced reliable and reproducible counts, comparable to the counting chamber. This experiment demonstrates that the faster, less technically demanding Coulter counter may be an alternative to determine numbers of leptospires.

Original languageEnglish
Pages (from-to)962-963
Number of pages2
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume73
Issue number5
DOIs
StatePublished - Nov 2005
Externally publishedYes

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