Short report: Enumerating leptospires using the Coulter counter

Christina M. Humberd; Clinton K. Murray; Saundra K. Stuart; Barbara A. Reeb; Duane R. Hospenthal

doi:10.4269/ajtmh.2005.73.962

Short report: Enumerating leptospires using the Coulter counter

Christina M. Humberd^*, Clinton K. Murray, Saundra K. Stuart, Barbara A. Reeb, Duane R. Hospenthal

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

The currently accepted gold standard for enumeration of leptospires is tabulation of organisms using a Petroff-Hausser counting chamber and dark-field microscopy, a technically demanding, time-consuming technique. Quantification of leptospires with a Coulter counter produced reliable and reproducible counts, comparable to the counting chamber. This experiment demonstrates that the faster, less technically demanding Coulter counter may be an alternative to determine numbers of leptospires.

Original language	English
Pages (from-to)	962-963
Number of pages	2
Journal	American Journal of Tropical Medicine and Hygiene
Volume	73
Issue number	5
DOIs	https://doi.org/10.4269/ajtmh.2005.73.962
State	Published - Nov 2005
Externally published	Yes

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10.4269/ajtmh.2005.73.962

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abstract = "The currently accepted gold standard for enumeration of leptospires is tabulation of organisms using a Petroff-Hausser counting chamber and dark-field microscopy, a technically demanding, time-consuming technique. Quantification of leptospires with a Coulter counter produced reliable and reproducible counts, comparable to the counting chamber. This experiment demonstrates that the faster, less technically demanding Coulter counter may be an alternative to determine numbers of leptospires.",

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AU - Hospenthal, Duane R.

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Short report: Enumerating leptospires using the Coulter counter

Abstract

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