TY - JOUR
T1 - Should Surgeons Clone Genes?
T2 - The Strategy Behind the Cloning of the Human Inducible Nitric Oxide Synthase Gene
AU - Geller, David A.
AU - Billiar, Timothy R.
N1 - Funding Information:
Accepted for publication August 8, 1993. Thisworkwas supported by grants GM-44100 (Dr Bil- liar) and GM-37753 (Richard L. Simmons, MD)from the NationalInstitutes of Health, Bethesda, Md, grantDCB910003P (DrBillar)from thePittsburgh Supercomputing Center, the George H. A. Clowes, MD, FACS, Memorial Career Devel¬ opmentAward(DrBilliar)fromtheAmericanCollegeofSur¬ geons, Chicago, III, Society of University Surgeons/ WeEthiconFellowship thecontributionsallawardandthe(DrGeller). acknowledge of the original cloning paper. Reprints not available.
PY - 1993/11
Y1 - 1993/11
N2 - Nitric oxide (NO) is a recently recognized biological mediator that has triggered an explosion of scientific research over the last 5 years, resulting in NO being named “Molecule of the Year” by Science in 1992. Much of the excitement is due to the physiological and pathophysiological functions exerted by NO as a vasodilator, neurotransmitter, and antimicrobial effector molecule. The purpose of this review is to describe the cloning of the inducible NO synthase gene from human hepatocytes, with particular emphasis on the importance to surgeons of cloning genes and on the utility of applying modern molecular biology to the study of diseases relevant to surgical practice. Overall cloning techniques and the specific strategy used to clone the human inducible NO synthase gene are reviewed. In addition, applications of gene cloning to clinical surgery are discussed. Isolating the inducible human NO synthase gene has provided important information regarding the regulation of induced NO synthesis in human cells and has provided a critical tool for further studies to define the role of NO in normal and disease processes.
AB - Nitric oxide (NO) is a recently recognized biological mediator that has triggered an explosion of scientific research over the last 5 years, resulting in NO being named “Molecule of the Year” by Science in 1992. Much of the excitement is due to the physiological and pathophysiological functions exerted by NO as a vasodilator, neurotransmitter, and antimicrobial effector molecule. The purpose of this review is to describe the cloning of the inducible NO synthase gene from human hepatocytes, with particular emphasis on the importance to surgeons of cloning genes and on the utility of applying modern molecular biology to the study of diseases relevant to surgical practice. Overall cloning techniques and the specific strategy used to clone the human inducible NO synthase gene are reviewed. In addition, applications of gene cloning to clinical surgery are discussed. Isolating the inducible human NO synthase gene has provided important information regarding the regulation of induced NO synthesis in human cells and has provided a critical tool for further studies to define the role of NO in normal and disease processes.
UR - http://www.scopus.com/inward/record.url?scp=0027358674&partnerID=8YFLogxK
U2 - 10.1001/archsurg.1993.01420230040006
DO - 10.1001/archsurg.1993.01420230040006
M3 - Article
C2 - 7694564
AN - SCOPUS:0027358674
SN - 0004-0010
VL - 128
SP - 1212
EP - 1220
JO - Archives of Surgery
JF - Archives of Surgery
IS - 11
ER -