TY - JOUR
T1 - Silver-doped bioactive glass particles for in vivo bone tissue regeneration and enhanced methicillin-resistant Staphylococcus aureus (MRSA) inhibition
AU - Pajares-Chamorro, Natalia
AU - Wagley, Yadav
AU - Maduka, Chima V.
AU - Youngstrom, Daniel W.
AU - Yeger, Alyssa
AU - Badylak, Stephen F.
AU - Hammer, Neal D.
AU - Hankenson, Kurt
AU - Chatzistavrou, Xanthippi
N1 - Publisher Copyright:
© 2020 Elsevier B.V.
PY - 2021/1
Y1 - 2021/1
N2 - Infection is a significant risk factor for failed healing of bone and other tissues. We have developed a sol-gel (solution-gelation) derived bioactive glass doped with silver ions (Ag-BG), tailored to provide non-cytotoxic antibacterial activity while significantly enhancing osteoblast-lineage cell growth in vitro and bone regeneration in vivo. Our objective was to engineer a biomaterial that combats bacterial infection while maintaining the capability to promote bone growth. We observed that Ag-BG inhibits bacterial growth and potentiates the efficacy of conventional antibiotic treatment. Ag-BG microparticles enhance cell proliferation and osteogenic differentiation in human bone marrow stromal cells (hBMSC) in vitro. Moreover, in vivo tests using a calvarial defect model in mice demonstrated that Ag-BG microparticles induce bone regeneration. This novel system with dual biological and advanced antibacterial properties is a promising therapeutic for combating resistant bacteria while triggering new bone formation.
AB - Infection is a significant risk factor for failed healing of bone and other tissues. We have developed a sol-gel (solution-gelation) derived bioactive glass doped with silver ions (Ag-BG), tailored to provide non-cytotoxic antibacterial activity while significantly enhancing osteoblast-lineage cell growth in vitro and bone regeneration in vivo. Our objective was to engineer a biomaterial that combats bacterial infection while maintaining the capability to promote bone growth. We observed that Ag-BG inhibits bacterial growth and potentiates the efficacy of conventional antibiotic treatment. Ag-BG microparticles enhance cell proliferation and osteogenic differentiation in human bone marrow stromal cells (hBMSC) in vitro. Moreover, in vivo tests using a calvarial defect model in mice demonstrated that Ag-BG microparticles induce bone regeneration. This novel system with dual biological and advanced antibacterial properties is a promising therapeutic for combating resistant bacteria while triggering new bone formation.
KW - Calvarial bone growth
KW - Cell differentiation
KW - Cell viability
KW - Methicillin-resistant Staphylococcus aureus
KW - Silver doped bioactive glass
KW - Synergistic antibacterial properties
UR - http://www.scopus.com/inward/record.url?scp=85095867516&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2020.111693
DO - 10.1016/j.msec.2020.111693
M3 - Article
C2 - 33545854
AN - SCOPUS:85095867516
SN - 0928-4931
VL - 120
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
M1 - 111693
ER -