Silver-doped bioactive glass particles for in vivo bone tissue regeneration and enhanced methicillin-resistant Staphylococcus aureus (MRSA) inhibition

Natalia Pajares-Chamorro; Yadav Wagley; Chima V. Maduka; Daniel W. Youngstrom; Alyssa Yeger; Stephen F. Badylak; Neal D. Hammer; Kurt Hankenson; Xanthippi Chatzistavrou

doi:10.1016/j.msec.2020.111693

Silver-doped bioactive glass particles for in vivo bone tissue regeneration and enhanced methicillin-resistant Staphylococcus aureus (MRSA) inhibition

Natalia Pajares-Chamorro, Yadav Wagley, Chima V. Maduka, Daniel W. Youngstrom, Alyssa Yeger, Stephen F. Badylak, Neal D. Hammer, Kurt Hankenson, Xanthippi Chatzistavrou^*

^*Corresponding author for this work

Surgery

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Infection is a significant risk factor for failed healing of bone and other tissues. We have developed a sol-gel (solution-gelation) derived bioactive glass doped with silver ions (Ag-BG), tailored to provide non-cytotoxic antibacterial activity while significantly enhancing osteoblast-lineage cell growth in vitro and bone regeneration in vivo. Our objective was to engineer a biomaterial that combats bacterial infection while maintaining the capability to promote bone growth. We observed that Ag-BG inhibits bacterial growth and potentiates the efficacy of conventional antibiotic treatment. Ag-BG microparticles enhance cell proliferation and osteogenic differentiation in human bone marrow stromal cells (hBMSC) in vitro. Moreover, in vivo tests using a calvarial defect model in mice demonstrated that Ag-BG microparticles induce bone regeneration. This novel system with dual biological and advanced antibacterial properties is a promising therapeutic for combating resistant bacteria while triggering new bone formation.

Original language	English
Article number	111693
Journal	Materials Science and Engineering C
Volume	120
DOIs	https://doi.org/10.1016/j.msec.2020.111693
State	Published - Jan 2021

Keywords

Calvarial bone growth
Cell differentiation
Cell viability
Methicillin-resistant Staphylococcus aureus
Silver doped bioactive glass
Synergistic antibacterial properties

Access to Document

10.1016/j.msec.2020.111693

Cite this

Pajares-Chamorro, N., Wagley, Y., Maduka, C. V., Youngstrom, D. W., Yeger, A., Badylak, S. F., Hammer, N. D., Hankenson, K., & Chatzistavrou, X. (2021). Silver-doped bioactive glass particles for in vivo bone tissue regeneration and enhanced methicillin-resistant Staphylococcus aureus (MRSA) inhibition. Materials Science and Engineering C, 120, Article 111693. https://doi.org/10.1016/j.msec.2020.111693

@article{8a6315692c024f819333136b8394d420,

title = "Silver-doped bioactive glass particles for in vivo bone tissue regeneration and enhanced methicillin-resistant Staphylococcus aureus (MRSA) inhibition",

abstract = "Infection is a significant risk factor for failed healing of bone and other tissues. We have developed a sol-gel (solution-gelation) derived bioactive glass doped with silver ions (Ag-BG), tailored to provide non-cytotoxic antibacterial activity while significantly enhancing osteoblast-lineage cell growth in vitro and bone regeneration in vivo. Our objective was to engineer a biomaterial that combats bacterial infection while maintaining the capability to promote bone growth. We observed that Ag-BG inhibits bacterial growth and potentiates the efficacy of conventional antibiotic treatment. Ag-BG microparticles enhance cell proliferation and osteogenic differentiation in human bone marrow stromal cells (hBMSC) in vitro. Moreover, in vivo tests using a calvarial defect model in mice demonstrated that Ag-BG microparticles induce bone regeneration. This novel system with dual biological and advanced antibacterial properties is a promising therapeutic for combating resistant bacteria while triggering new bone formation.",

keywords = "Calvarial bone growth, Cell differentiation, Cell viability, Methicillin-resistant Staphylococcus aureus, Silver doped bioactive glass, Synergistic antibacterial properties",

author = "Natalia Pajares-Chamorro and Yadav Wagley and Maduka, {Chima V.} and Youngstrom, {Daniel W.} and Alyssa Yeger and Badylak, {Stephen F.} and Hammer, {Neal D.} and Kurt Hankenson and Xanthippi Chatzistavrou",

note = "Publisher Copyright: {\textcopyright} 2020 Elsevier B.V.",

year = "2021",

month = jan,

doi = "10.1016/j.msec.2020.111693",

language = "English",

volume = "120",

journal = "Materials Science and Engineering C",

issn = "0928-4931",

publisher = "Elsevier B.V.",

}

Pajares-Chamorro, N, Wagley, Y, Maduka, CV, Youngstrom, DW, Yeger, A, Badylak, SF, Hammer, ND, Hankenson, K & Chatzistavrou, X 2021, 'Silver-doped bioactive glass particles for in vivo bone tissue regeneration and enhanced methicillin-resistant Staphylococcus aureus (MRSA) inhibition', Materials Science and Engineering C, vol. 120, 111693. https://doi.org/10.1016/j.msec.2020.111693

TY - JOUR

T1 - Silver-doped bioactive glass particles for in vivo bone tissue regeneration and enhanced methicillin-resistant Staphylococcus aureus (MRSA) inhibition

AU - Pajares-Chamorro, Natalia

AU - Wagley, Yadav

AU - Maduka, Chima V.

AU - Youngstrom, Daniel W.

AU - Yeger, Alyssa

AU - Badylak, Stephen F.

AU - Hammer, Neal D.

AU - Hankenson, Kurt

AU - Chatzistavrou, Xanthippi

PY - 2021/1

Y1 - 2021/1

N2 - Infection is a significant risk factor for failed healing of bone and other tissues. We have developed a sol-gel (solution-gelation) derived bioactive glass doped with silver ions (Ag-BG), tailored to provide non-cytotoxic antibacterial activity while significantly enhancing osteoblast-lineage cell growth in vitro and bone regeneration in vivo. Our objective was to engineer a biomaterial that combats bacterial infection while maintaining the capability to promote bone growth. We observed that Ag-BG inhibits bacterial growth and potentiates the efficacy of conventional antibiotic treatment. Ag-BG microparticles enhance cell proliferation and osteogenic differentiation in human bone marrow stromal cells (hBMSC) in vitro. Moreover, in vivo tests using a calvarial defect model in mice demonstrated that Ag-BG microparticles induce bone regeneration. This novel system with dual biological and advanced antibacterial properties is a promising therapeutic for combating resistant bacteria while triggering new bone formation.

AB - Infection is a significant risk factor for failed healing of bone and other tissues. We have developed a sol-gel (solution-gelation) derived bioactive glass doped with silver ions (Ag-BG), tailored to provide non-cytotoxic antibacterial activity while significantly enhancing osteoblast-lineage cell growth in vitro and bone regeneration in vivo. Our objective was to engineer a biomaterial that combats bacterial infection while maintaining the capability to promote bone growth. We observed that Ag-BG inhibits bacterial growth and potentiates the efficacy of conventional antibiotic treatment. Ag-BG microparticles enhance cell proliferation and osteogenic differentiation in human bone marrow stromal cells (hBMSC) in vitro. Moreover, in vivo tests using a calvarial defect model in mice demonstrated that Ag-BG microparticles induce bone regeneration. This novel system with dual biological and advanced antibacterial properties is a promising therapeutic for combating resistant bacteria while triggering new bone formation.

KW - Calvarial bone growth

KW - Cell differentiation

KW - Cell viability

KW - Methicillin-resistant Staphylococcus aureus

KW - Silver doped bioactive glass

KW - Synergistic antibacterial properties

UR - http://www.scopus.com/inward/record.url?scp=85095867516&partnerID=8YFLogxK

U2 - 10.1016/j.msec.2020.111693

DO - 10.1016/j.msec.2020.111693

M3 - Article

C2 - 33545854

AN - SCOPUS:85095867516

SN - 0928-4931

VL - 120

JO - Materials Science and Engineering C

JF - Materials Science and Engineering C

M1 - 111693

ER -

Silver-doped bioactive glass particles for in vivo bone tissue regeneration and enhanced methicillin-resistant Staphylococcus aureus (MRSA) inhibition

Abstract

Keywords

Access to Document

Fingerprint

Cite this