Abstract
Three separate studies were done to evaluate a new canine model of arterial thrombosis with endothelial injury. Endothelial injury was produced by exposing the luminal surface of a 2-cm segment of femoral artery to 100°C saline for 5 min. There was no disruption of proximal or distal blood flow with this model, and thrombolysis was continuously monitored by measuring 125I-labelled fibrin gamma emissions from the thrombus. Study No. 1 showed that complete endothelial denudation was achieved with this model. Study No. 2 demonstrated 1) adherence of the experimentally induced thrombus to subendothelial connective tissue, and 2) endogenous thrombolysis of approximately 9% during the initial 2 h after thrombus formation. Study No. 3 tested the usefulness of the model for evaluating the thrombolytic efficacy of urokinase. Urokinase (30,000 U Kg, bolus IV injection) caused 38 ± 5.4% thrombolysis within 90 min of drug administration versus 5.9 ± 2.4% for a saline-treated control group. We conclude that this model provides a technically simple and reproducible method for the laboratory investigation of thrombosis and thrombolysis in arteries with endothelial injury.
Original language | English |
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Pages (from-to) | 293-304 |
Number of pages | 12 |
Journal | Journal of Pharmacological Methods |
Volume | 19 |
Issue number | 4 |
DOIs | |
State | Published - Jul 1988 |
Externally published | Yes |
Keywords
- Arterial thrombosis
- Model
- Thrombolysis
- Urokinase