Simultaneous identification of multiple β-lactamases in Acinetobacter baumannii in relation to carbapenem and ceftazidime resistance, using liquid chromatography-tandem mass spectrometry

Hein Trip; Katrin Mende; Joanna A. Majchrzykiewicz-Koehorst; Norbert J.A. Sedee; Albert G. Hulst; Hugo Jan Jansen; Clinton K. Murray; Armand Paauw

doi:10.1128/JCM.00620-15

Simultaneous identification of multiple β-lactamases in Acinetobacter baumannii in relation to carbapenem and ceftazidime resistance, using liquid chromatography-tandem mass spectrometry

Hein Trip^*, Katrin Mende, Joanna A. Majchrzykiewicz-Koehorst, Norbert J.A. Sedee, Albert G. Hulst, Hugo Jan Jansen, Clinton K. Murray, Armand Paauw

^*Corresponding author for this work

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16 Scopus citations

Abstract

Shotgun proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was applied to detect β-lactamases in clinical Acinetobacter baumannii isolates. The correlation of the detection of β-lactamase proteins (rather than PCR detection of the corresponding genes) with the resistance phenotypes demonstrated an added value for LC-MS/MS in antimicrobial susceptibility testing.

Original language	English
Pages (from-to)	1927-1930
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	53
Issue number	6
DOIs	https://doi.org/10.1128/JCM.00620-15
State	Published - 1 Jun 2015
Externally published	Yes

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Trip, H., Mende, K., Majchrzykiewicz-Koehorst, J. A., Sedee, N. J. A., Hulst, A. G., Jansen, H. J., Murray, C. K., & Paauw, A. (2015). Simultaneous identification of multiple β-lactamases in Acinetobacter baumannii in relation to carbapenem and ceftazidime resistance, using liquid chromatography-tandem mass spectrometry. Journal of Clinical Microbiology, 53(6), 1927-1930. https://doi.org/10.1128/JCM.00620-15

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title = "Simultaneous identification of multiple β-lactamases in Acinetobacter baumannii in relation to carbapenem and ceftazidime resistance, using liquid chromatography-tandem mass spectrometry",

abstract = "Shotgun proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was applied to detect β-lactamases in clinical Acinetobacter baumannii isolates. The correlation of the detection of β-lactamase proteins (rather than PCR detection of the corresponding genes) with the resistance phenotypes demonstrated an added value for LC-MS/MS in antimicrobial susceptibility testing.",

author = "Hein Trip and Katrin Mende and Majchrzykiewicz-Koehorst, {Joanna A.} and Sedee, {Norbert J.A.} and Hulst, {Albert G.} and Jansen, {Hugo Jan} and Murray, {Clinton K.} and Armand Paauw",

year = "2015",

month = jun,

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doi = "10.1128/JCM.00620-15",

language = "English",

volume = "53",

pages = "1927--1930",

journal = "Journal of Clinical Microbiology",

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Trip, H, Mende, K, Majchrzykiewicz-Koehorst, JA, Sedee, NJA, Hulst, AG, Jansen, HJ, Murray, CK & Paauw, A 2015, 'Simultaneous identification of multiple β-lactamases in Acinetobacter baumannii in relation to carbapenem and ceftazidime resistance, using liquid chromatography-tandem mass spectrometry', Journal of Clinical Microbiology, vol. 53, no. 6, pp. 1927-1930. https://doi.org/10.1128/JCM.00620-15

Simultaneous identification of multiple β-lactamases in Acinetobacter baumannii in relation to carbapenem and ceftazidime resistance, using liquid chromatography-tandem mass spectrometry. / Trip, Hein; Mende, Katrin; Majchrzykiewicz-Koehorst, Joanna A. et al.
In: Journal of Clinical Microbiology, Vol. 53, No. 6, 01.06.2015, p. 1927-1930.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Simultaneous identification of multiple β-lactamases in Acinetobacter baumannii in relation to carbapenem and ceftazidime resistance, using liquid chromatography-tandem mass spectrometry

AU - Trip, Hein

AU - Mende, Katrin

AU - Majchrzykiewicz-Koehorst, Joanna A.

AU - Sedee, Norbert J.A.

AU - Hulst, Albert G.

AU - Jansen, Hugo Jan

AU - Murray, Clinton K.

AU - Paauw, Armand

PY - 2015/6/1

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N2 - Shotgun proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was applied to detect β-lactamases in clinical Acinetobacter baumannii isolates. The correlation of the detection of β-lactamase proteins (rather than PCR detection of the corresponding genes) with the resistance phenotypes demonstrated an added value for LC-MS/MS in antimicrobial susceptibility testing.

AB - Shotgun proteomics using liquid chromatography-tandem mass spectrometry (LC-MS/MS) was applied to detect β-lactamases in clinical Acinetobacter baumannii isolates. The correlation of the detection of β-lactamase proteins (rather than PCR detection of the corresponding genes) with the resistance phenotypes demonstrated an added value for LC-MS/MS in antimicrobial susceptibility testing.

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SN - 0095-1137

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IS - 6

ER -

Simultaneous identification of multiple β-lactamases in Acinetobacter baumannii in relation to carbapenem and ceftazidime resistance, using liquid chromatography-tandem mass spectrometry

Abstract

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