Small-Volume Resuscitation with the Hemoglobin Substitute HBOC-201: Effect on Brain Tissue Oxygenation

Geoffrey T. Manley; J. Claude Hemphill; Diane Morabito; Vanessa Erickson; John J. Holcroft; Nakita Derugin; M. Margaret Knudson

doi:10.1007/978-1-4615-0075-9_29

Small-Volume Resuscitation with the Hemoglobin Substitute HBOC-201: Effect on Brain Tissue Oxygenation

Geoffrey T. Manley^*, J. Claude Hemphill, Diane Morabito, Vanessa Erickson, John J. Holcroft, Nakita Derugin, M. Margaret Knudson

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

OBJECTIVES: To investigate the effects of small-volume resuscitation with a hemoglobin based oxygen carrier on brain tissue oxygen tension (PbrO ₂) in hemorrhaged swine. METHODS: Clark-type electrodes were inserted into the brain tissue of 6 swine to measure PbrO₂ directly. Swine were hemorrhaged to a MAP of 40 mm Hg for 20 minutes. Resuscitation was performed with a bolus infusion of HBOC-201 (6 cc/kg; Biopure Corp.) and high-flow oxygen (100%). Swine were observed for an additional 2 hours. RESULTS: PbrO₂ prior to hemorrhage was 48.7 ± 4.7 mm Hg with 100% inspired oxygen. PbrO₂ rapidly declined to 7.6 ± 5.3 mm Hg in response to hemorrhage. Small-volume resuscitation with HBOC-201 and high-flow oxygen resulted in a significant increase (p<0.001) in PbrO2 to 44.6 ± 8.1 mm Hg. MAP was also significantly increased to 84% of baseline. These elevations were sustained during the observation period. CONCLUSIONS: Resuscitation with HBOC-201 can restore and sustain cerebral oxygenation and MAP. These results suggest that a small-volume bolus of HBOC-201 may provide adequate oxygen and pressure support during the initial management of hemorrhage.

Original language	English
Pages (from-to)	311-317
Number of pages	7
Journal	Advances in Experimental Medicine and Biology
Volume	530
DOIs	https://doi.org/10.1007/978-1-4615-0075-9_29
State	Published - 2003
Externally published	Yes

Keywords

Blood substitutes
Cerebral oxygenation
Cerebral resuscitation
Hemorrhage

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@article{3670e6b7b6a54cdea91ff2af1effa256,

title = "Small-Volume Resuscitation with the Hemoglobin Substitute HBOC-201: Effect on Brain Tissue Oxygenation",

abstract = "OBJECTIVES: To investigate the effects of small-volume resuscitation with a hemoglobin based oxygen carrier on brain tissue oxygen tension (PbrO 2) in hemorrhaged swine. METHODS: Clark-type electrodes were inserted into the brain tissue of 6 swine to measure PbrO2 directly. Swine were hemorrhaged to a MAP of 40 mm Hg for 20 minutes. Resuscitation was performed with a bolus infusion of HBOC-201 (6 cc/kg; Biopure Corp.) and high-flow oxygen (100%). Swine were observed for an additional 2 hours. RESULTS: PbrO2 prior to hemorrhage was 48.7 ± 4.7 mm Hg with 100% inspired oxygen. PbrO2 rapidly declined to 7.6 ± 5.3 mm Hg in response to hemorrhage. Small-volume resuscitation with HBOC-201 and high-flow oxygen resulted in a significant increase (p<0.001) in PbrO2 to 44.6 ± 8.1 mm Hg. MAP was also significantly increased to 84% of baseline. These elevations were sustained during the observation period. CONCLUSIONS: Resuscitation with HBOC-201 can restore and sustain cerebral oxygenation and MAP. These results suggest that a small-volume bolus of HBOC-201 may provide adequate oxygen and pressure support during the initial management of hemorrhage.",

keywords = "Blood substitutes, Cerebral oxygenation, Cerebral resuscitation, Hemorrhage",

author = "Manley, {Geoffrey T.} and Hemphill, {J. Claude} and Diane Morabito and Vanessa Erickson and Holcroft, {John J.} and Nakita Derugin and Knudson, {M. Margaret}",

year = "2003",

doi = "10.1007/978-1-4615-0075-9_29",

language = "English",

volume = "530",

pages = "311--317",

journal = "Advances in Experimental Medicine and Biology",

issn = "0065-2598",

}

TY - JOUR

T1 - Small-Volume Resuscitation with the Hemoglobin Substitute HBOC-201

T2 - Effect on Brain Tissue Oxygenation

AU - Manley, Geoffrey T.

AU - Hemphill, J. Claude

AU - Morabito, Diane

AU - Erickson, Vanessa

AU - Holcroft, John J.

AU - Derugin, Nakita

AU - Knudson, M. Margaret

PY - 2003

Y1 - 2003

N2 - OBJECTIVES: To investigate the effects of small-volume resuscitation with a hemoglobin based oxygen carrier on brain tissue oxygen tension (PbrO 2) in hemorrhaged swine. METHODS: Clark-type electrodes were inserted into the brain tissue of 6 swine to measure PbrO2 directly. Swine were hemorrhaged to a MAP of 40 mm Hg for 20 minutes. Resuscitation was performed with a bolus infusion of HBOC-201 (6 cc/kg; Biopure Corp.) and high-flow oxygen (100%). Swine were observed for an additional 2 hours. RESULTS: PbrO2 prior to hemorrhage was 48.7 ± 4.7 mm Hg with 100% inspired oxygen. PbrO2 rapidly declined to 7.6 ± 5.3 mm Hg in response to hemorrhage. Small-volume resuscitation with HBOC-201 and high-flow oxygen resulted in a significant increase (p<0.001) in PbrO2 to 44.6 ± 8.1 mm Hg. MAP was also significantly increased to 84% of baseline. These elevations were sustained during the observation period. CONCLUSIONS: Resuscitation with HBOC-201 can restore and sustain cerebral oxygenation and MAP. These results suggest that a small-volume bolus of HBOC-201 may provide adequate oxygen and pressure support during the initial management of hemorrhage.

AB - OBJECTIVES: To investigate the effects of small-volume resuscitation with a hemoglobin based oxygen carrier on brain tissue oxygen tension (PbrO 2) in hemorrhaged swine. METHODS: Clark-type electrodes were inserted into the brain tissue of 6 swine to measure PbrO2 directly. Swine were hemorrhaged to a MAP of 40 mm Hg for 20 minutes. Resuscitation was performed with a bolus infusion of HBOC-201 (6 cc/kg; Biopure Corp.) and high-flow oxygen (100%). Swine were observed for an additional 2 hours. RESULTS: PbrO2 prior to hemorrhage was 48.7 ± 4.7 mm Hg with 100% inspired oxygen. PbrO2 rapidly declined to 7.6 ± 5.3 mm Hg in response to hemorrhage. Small-volume resuscitation with HBOC-201 and high-flow oxygen resulted in a significant increase (p<0.001) in PbrO2 to 44.6 ± 8.1 mm Hg. MAP was also significantly increased to 84% of baseline. These elevations were sustained during the observation period. CONCLUSIONS: Resuscitation with HBOC-201 can restore and sustain cerebral oxygenation and MAP. These results suggest that a small-volume bolus of HBOC-201 may provide adequate oxygen and pressure support during the initial management of hemorrhage.

KW - Blood substitutes

KW - Cerebral oxygenation

KW - Cerebral resuscitation

KW - Hemorrhage

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DO - 10.1007/978-1-4615-0075-9_29

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JO - Advances in Experimental Medicine and Biology

JF - Advances in Experimental Medicine and Biology

ER -

Small-Volume Resuscitation with the Hemoglobin Substitute HBOC-201: Effect on Brain Tissue Oxygenation

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Keywords

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