Small-Volume Resuscitation with the Hemoglobin Substitute HBOC-201: Effect on Brain Tissue Oxygenation

Geoffrey T. Manley*, J. Claude Hemphill, Diane Morabito, Vanessa Erickson, John J. Holcroft, Nakita Derugin, M. Margaret Knudson

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

14 Scopus citations


OBJECTIVES: To investigate the effects of small-volume resuscitation with a hemoglobin based oxygen carrier on brain tissue oxygen tension (PbrO 2) in hemorrhaged swine. METHODS: Clark-type electrodes were inserted into the brain tissue of 6 swine to measure PbrO2 directly. Swine were hemorrhaged to a MAP of 40 mm Hg for 20 minutes. Resuscitation was performed with a bolus infusion of HBOC-201 (6 cc/kg; Biopure Corp.) and high-flow oxygen (100%). Swine were observed for an additional 2 hours. RESULTS: PbrO2 prior to hemorrhage was 48.7 ± 4.7 mm Hg with 100% inspired oxygen. PbrO2 rapidly declined to 7.6 ± 5.3 mm Hg in response to hemorrhage. Small-volume resuscitation with HBOC-201 and high-flow oxygen resulted in a significant increase (p<0.001) in PbrO2 to 44.6 ± 8.1 mm Hg. MAP was also significantly increased to 84% of baseline. These elevations were sustained during the observation period. CONCLUSIONS: Resuscitation with HBOC-201 can restore and sustain cerebral oxygenation and MAP. These results suggest that a small-volume bolus of HBOC-201 may provide adequate oxygen and pressure support during the initial management of hemorrhage.

Original languageEnglish
Pages (from-to)311-317
Number of pages7
JournalAdvances in Experimental Medicine and Biology
StatePublished - 2003
Externally publishedYes


  • Blood substitutes
  • Cerebral oxygenation
  • Cerebral resuscitation
  • Hemorrhage


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