Smart hydrogel particles: Biomarker harvesting: One-step affinity purification, size exclusion, and protection against degradation

Alessandra Luchini, David H. Geho, Barney Bishops, Duy Tran, Cassandra Xia, Robert L. Dufour, Clinton D. Jones, Virginia Espina, Alexis Patanarut, Weidong Zhou, Mark M. Ross, Alessandra Tessitore, Emanuel F. Petricoin, Lance A. Liotta*

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

175 Scopus citations

Abstract

Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions within minutes, in one step, in solution: (a) molecular size sieving, (b) affinity capture of all solution-phase target molecules, and (c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis.

Original languageEnglish
Pages (from-to)350-361
Number of pages12
JournalNano Letters
Volume8
Issue number1
DOIs
StatePublished - Jan 2008
Externally publishedYes

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