Smart hydrogel particles: Biomarker harvesting: One-step affinity purification, size exclusion, and protection against degradation

Alessandra Luchini; David H. Geho; Barney Bishops; Duy Tran; Cassandra Xia; Robert L. Dufour; Clinton D. Jones; Virginia Espina; Alexis Patanarut; Weidong Zhou; Mark M. Ross; Alessandra Tessitore; Emanuel F. Petricoin; Lance A. Liotta

doi:10.1021/nl072174l

Smart hydrogel particles: Biomarker harvesting: One-step affinity purification, size exclusion, and protection against degradation

Alessandra Luchini, David H. Geho, Barney Bishops, Duy Tran, Cassandra Xia, Robert L. Dufour, Clinton D. Jones, Virginia Espina, Alexis Patanarut, Weidong Zhou, Mark M. Ross, Alessandra Tessitore, Emanuel F. Petricoin, Lance A. Liotta^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

179 Scopus citations

Abstract

Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions within minutes, in one step, in solution: (a) molecular size sieving, (b) affinity capture of all solution-phase target molecules, and (c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis.

Original language	English
Pages (from-to)	350-361
Number of pages	12
Journal	Nano Letters
Volume	8
Issue number	1
DOIs	https://doi.org/10.1021/nl072174l
State	Published - Jan 2008
Externally published	Yes

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Luchini, A., Geho, D. H., Bishops, B., Tran, D., Xia, C., Dufour, R. L., Jones, C. D., Espina, V., Patanarut, A., Zhou, W., Ross, M. M., Tessitore, A., Petricoin, E. F., & Liotta, L. A. (2008). Smart hydrogel particles: Biomarker harvesting: One-step affinity purification, size exclusion, and protection against degradation. Nano Letters, 8(1), 350-361. https://doi.org/10.1021/nl072174l

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title = "Smart hydrogel particles: Biomarker harvesting: One-step affinity purification, size exclusion, and protection against degradation",

abstract = "Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions within minutes, in one step, in solution: (a) molecular size sieving, (b) affinity capture of all solution-phase target molecules, and (c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis.",

author = "Alessandra Luchini and Geho, {David H.} and Barney Bishops and Duy Tran and Cassandra Xia and Dufour, {Robert L.} and Jones, {Clinton D.} and Virginia Espina and Alexis Patanarut and Weidong Zhou and Ross, {Mark M.} and Alessandra Tessitore and Petricoin, {Emanuel F.} and Liotta, {Lance A.}",

year = "2008",

month = jan,

doi = "10.1021/nl072174l",

language = "English",

volume = "8",

pages = "350--361",

journal = "Nano Letters",

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Luchini, A, Geho, DH, Bishops, B, Tran, D, Xia, C, Dufour, RL, Jones, CD, Espina, V, Patanarut, A, Zhou, W, Ross, MM, Tessitore, A, Petricoin, EF & Liotta, LA 2008, 'Smart hydrogel particles: Biomarker harvesting: One-step affinity purification, size exclusion, and protection against degradation', Nano Letters, vol. 8, no. 1, pp. 350-361. https://doi.org/10.1021/nl072174l

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T1 - Smart hydrogel particles

T2 - Biomarker harvesting: One-step affinity purification, size exclusion, and protection against degradation

AU - Luchini, Alessandra

AU - Geho, David H.

AU - Bishops, Barney

AU - Tran, Duy

AU - Xia, Cassandra

AU - Dufour, Robert L.

AU - Jones, Clinton D.

AU - Espina, Virginia

AU - Patanarut, Alexis

AU - Zhou, Weidong

AU - Ross, Mark M.

AU - Tessitore, Alessandra

AU - Petricoin, Emanuel F.

AU - Liotta, Lance A.

PY - 2008/1

Y1 - 2008/1

N2 - Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions within minutes, in one step, in solution: (a) molecular size sieving, (b) affinity capture of all solution-phase target molecules, and (c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis.

AB - Disease-associated blood biomarkers exist in exceedingly low concentrations within complex mixtures of high-abundance proteins such as albumin. We have introduced an affinity bait molecule into N-isopropylacrylamide to produce a particle that will perform three independent functions within minutes, in one step, in solution: (a) molecular size sieving, (b) affinity capture of all solution-phase target molecules, and (c) complete protection of harvested proteins from enzymatic degradation. The captured analytes can be readily electroeluted for analysis.

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JO - Nano Letters

JF - Nano Letters

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ER -

Smart hydrogel particles: Biomarker harvesting: One-step affinity purification, size exclusion, and protection against degradation

Abstract

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