Solid-phase antibody capture hemadsorption assay for detection of hepatitis A virus immunoglobulin M antibodies

P. L. Summers; D. R. Dubois; W. H. Cohen; P. O. Macarthy; L. N. Binn; M. H. Sjogren; R. Snitbhan; B. L. Innis; K. H. Eckels

doi:10.1128/jcm.31.5.1299-1302.1993

Solid-phase antibody capture hemadsorption assay for detection of hepatitis A virus immunoglobulin M antibodies

P. L. Summers^*, D. R. Dubois, W. H. Cohen, P. O. Macarthy, L. N. Binn, M. H. Sjogren, R. Snitbhan, B. L. Innis, K. H. Eckels

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

A solid-phase antibody capture hemadsorption (SPACH) assay was developed to detect hepatitis A virus (HAV)-specific immunoglobulin M (IgM) antibodies in sera from humans recently infected with hepatitis. The assay is performed with microtiter plates coated with anti-human IgM antibodies to capture IgM antibodies from the test sera. HAV-specific IgM antibody is detected by the addition of HAV hemagglutinating antigen and goose erythrocytes. Hemadsorption of erythrocytes to antigen-antibody complexes attached to the solid phase indicate the presence of IgM antibodies. The SPACH assay was compared to a commercial radioimmunoassay and was found to be equally or more sensitive and specific for the detection of HAV IgM antibodies. The SPACH assay is an alternative, rapid assay that doesn't require hazardous substrates or radioactivity for the detection of HAV-specific antibodies.

Original language	English
Pages (from-to)	1299-1302
Number of pages	4
Journal	Journal of Clinical Microbiology
Volume	31
Issue number	5
DOIs	https://doi.org/10.1128/jcm.31.5.1299-1302.1993
State	Published - 1993
Externally published	Yes

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10.1128/jcm.31.5.1299-1302.1993

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title = "Solid-phase antibody capture hemadsorption assay for detection of hepatitis A virus immunoglobulin M antibodies",

abstract = "A solid-phase antibody capture hemadsorption (SPACH) assay was developed to detect hepatitis A virus (HAV)-specific immunoglobulin M (IgM) antibodies in sera from humans recently infected with hepatitis. The assay is performed with microtiter plates coated with anti-human IgM antibodies to capture IgM antibodies from the test sera. HAV-specific IgM antibody is detected by the addition of HAV hemagglutinating antigen and goose erythrocytes. Hemadsorption of erythrocytes to antigen-antibody complexes attached to the solid phase indicate the presence of IgM antibodies. The SPACH assay was compared to a commercial radioimmunoassay and was found to be equally or more sensitive and specific for the detection of HAV IgM antibodies. The SPACH assay is an alternative, rapid assay that doesn't require hazardous substrates or radioactivity for the detection of HAV-specific antibodies.",

author = "Summers, {P. L.} and Dubois, {D. R.} and Cohen, {W. H.} and Macarthy, {P. O.} and Binn, {L. N.} and Sjogren, {M. H.} and R. Snitbhan and Innis, {B. L.} and Eckels, {K. H.}",

year = "1993",

doi = "10.1128/jcm.31.5.1299-1302.1993",

language = "English",

volume = "31",

pages = "1299--1302",

journal = "Journal of Clinical Microbiology",

issn = "0095-1137",

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T1 - Solid-phase antibody capture hemadsorption assay for detection of hepatitis A virus immunoglobulin M antibodies

AU - Summers, P. L.

AU - Dubois, D. R.

AU - Cohen, W. H.

AU - Macarthy, P. O.

AU - Binn, L. N.

AU - Sjogren, M. H.

AU - Snitbhan, R.

AU - Innis, B. L.

AU - Eckels, K. H.

PY - 1993

Y1 - 1993

N2 - A solid-phase antibody capture hemadsorption (SPACH) assay was developed to detect hepatitis A virus (HAV)-specific immunoglobulin M (IgM) antibodies in sera from humans recently infected with hepatitis. The assay is performed with microtiter plates coated with anti-human IgM antibodies to capture IgM antibodies from the test sera. HAV-specific IgM antibody is detected by the addition of HAV hemagglutinating antigen and goose erythrocytes. Hemadsorption of erythrocytes to antigen-antibody complexes attached to the solid phase indicate the presence of IgM antibodies. The SPACH assay was compared to a commercial radioimmunoassay and was found to be equally or more sensitive and specific for the detection of HAV IgM antibodies. The SPACH assay is an alternative, rapid assay that doesn't require hazardous substrates or radioactivity for the detection of HAV-specific antibodies.

AB - A solid-phase antibody capture hemadsorption (SPACH) assay was developed to detect hepatitis A virus (HAV)-specific immunoglobulin M (IgM) antibodies in sera from humans recently infected with hepatitis. The assay is performed with microtiter plates coated with anti-human IgM antibodies to capture IgM antibodies from the test sera. HAV-specific IgM antibody is detected by the addition of HAV hemagglutinating antigen and goose erythrocytes. Hemadsorption of erythrocytes to antigen-antibody complexes attached to the solid phase indicate the presence of IgM antibodies. The SPACH assay was compared to a commercial radioimmunoassay and was found to be equally or more sensitive and specific for the detection of HAV IgM antibodies. The SPACH assay is an alternative, rapid assay that doesn't require hazardous substrates or radioactivity for the detection of HAV-specific antibodies.

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DO - 10.1128/jcm.31.5.1299-1302.1993

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EP - 1302

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

IS - 5

ER -

Solid-phase antibody capture hemadsorption assay for detection of hepatitis A virus immunoglobulin M antibodies

Abstract

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