Spontaneously increased production of nitric oxide and aberrant expression of the inducible nitric oxide synthase in vivo in the transforming growth factor β1 null mouse

Yoram Vodovotz*, Andrew G. Geiser, Louis Chesler, John J. Letterio, Andrew Campbell, M. Scott Lucia, Michael B. Sporn, Anita B. Roberts

*Corresponding author for this work

Research output: Contribution to journalArticlepeer-review

93 Scopus citations

Abstract

Transforming growth factor β1 null mice (TGF-β1(-/-)) suffer from multifocal inflammation and die by 3-4 wk of age. In these mice, levels of nitric oxide (NO) reaction products in serum are elevated approximately fourfold over levels m controls, peaking at 15-17 d of life. Short-term treatment of TGF-β1(-/-) mice with N(G)-monomethyl-L-arginine suppressed this elevated production of NO. Expression of inducible NO synthase (iNOS) mRNA and protein is increased in the kidney and heart of TGF-β1(-/-) mice. These findings demonstrate that TGF-β1 negatively regulates iNOS expression in vivo, as had been inferred from mechanistic studies on the control of iNOS expression by TGF-β1 in vitro.

Original languageEnglish
Pages (from-to)2337-2342
Number of pages6
JournalJournal of Experimental Medicine
Volume183
Issue number5
DOIs
StatePublished - 1 May 1996
Externally publishedYes

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