Strain-dependent CNS dissemination in guinea pigs after Mycobacterium tuberculosis aerosol challenge

Nicholas A. Be; Lee G. Klinkenberg; William R. Bishai; Petros C. Karakousis; Sanjay K. Jain

doi:10.1016/j.tube.2011.07.003

Strain-dependent CNS dissemination in guinea pigs after Mycobacterium tuberculosis aerosol challenge

Nicholas A. Be, Lee G. Klinkenberg, William R. Bishai, Petros C. Karakousis, Sanjay K. Jain^*

^*Corresponding author for this work

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Clinical reports suggest an association of distinct Mycobacterium tuberculosis strains with CNS disease. We therefore examined CNS dissemination by different laboratory strains (two M. tuberculosis H37Rv, one CDC1551) in a guinea pig aerosol infection model. Although all strains grew exponentially in lungs, with similar bacterial burdens at the time of extrapulmonary dissemination, M. tuberculosis CDC1551 disseminated to the CNS significantly more than the H37Rv strains. No CNS lesions were observed throughout the study, with only a modest cytokine response. These data suggest that M. tuberculosis may have virulence factors that promote CNS dissemination, distinct from those required for pulmonary TB.

Original language	English
Pages (from-to)	386-389
Number of pages	4
Journal	Tuberculosis
Volume	91
Issue number	5
DOIs	https://doi.org/10.1016/j.tube.2011.07.003
State	Published - Sep 2011
Externally published	Yes

Keywords

Central nervous system
Dissemination
Guinea pig
Meningitis
Tuberculosis

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title = "Strain-dependent CNS dissemination in guinea pigs after Mycobacterium tuberculosis aerosol challenge",

abstract = "Clinical reports suggest an association of distinct Mycobacterium tuberculosis strains with CNS disease. We therefore examined CNS dissemination by different laboratory strains (two M. tuberculosis H37Rv, one CDC1551) in a guinea pig aerosol infection model. Although all strains grew exponentially in lungs, with similar bacterial burdens at the time of extrapulmonary dissemination, M. tuberculosis CDC1551 disseminated to the CNS significantly more than the H37Rv strains. No CNS lesions were observed throughout the study, with only a modest cytokine response. These data suggest that M. tuberculosis may have virulence factors that promote CNS dissemination, distinct from those required for pulmonary TB.",

keywords = "Central nervous system, Dissemination, Guinea pig, Meningitis, Tuberculosis",

author = "Be, {Nicholas A.} and Klinkenberg, {Lee G.} and Bishai, {William R.} and Karakousis, {Petros C.} and Jain, {Sanjay K.}",

note = "Funding Information: Financial support was provided by Bill and Melinda Gates Foundation #48793 and #42851 , NIH Director's New Innovator Award OD006492, CFAR Pilot Developmental Program (Johns Hopkins), and NIH contract and grant N01 30036 and AI79590 respectively. Financial sponsors had no role in the study design, collection, analysis, or interpretation of data. ",

year = "2011",

month = sep,

doi = "10.1016/j.tube.2011.07.003",

language = "English",

volume = "91",

pages = "386--389",

journal = "Tuberculosis",

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T1 - Strain-dependent CNS dissemination in guinea pigs after Mycobacterium tuberculosis aerosol challenge

AU - Be, Nicholas A.

AU - Klinkenberg, Lee G.

AU - Bishai, William R.

AU - Karakousis, Petros C.

AU - Jain, Sanjay K.

N1 - Funding Information: Financial support was provided by Bill and Melinda Gates Foundation #48793 and #42851 , NIH Director's New Innovator Award OD006492, CFAR Pilot Developmental Program (Johns Hopkins), and NIH contract and grant N01 30036 and AI79590 respectively. Financial sponsors had no role in the study design, collection, analysis, or interpretation of data.

PY - 2011/9

Y1 - 2011/9

N2 - Clinical reports suggest an association of distinct Mycobacterium tuberculosis strains with CNS disease. We therefore examined CNS dissemination by different laboratory strains (two M. tuberculosis H37Rv, one CDC1551) in a guinea pig aerosol infection model. Although all strains grew exponentially in lungs, with similar bacterial burdens at the time of extrapulmonary dissemination, M. tuberculosis CDC1551 disseminated to the CNS significantly more than the H37Rv strains. No CNS lesions were observed throughout the study, with only a modest cytokine response. These data suggest that M. tuberculosis may have virulence factors that promote CNS dissemination, distinct from those required for pulmonary TB.

AB - Clinical reports suggest an association of distinct Mycobacterium tuberculosis strains with CNS disease. We therefore examined CNS dissemination by different laboratory strains (two M. tuberculosis H37Rv, one CDC1551) in a guinea pig aerosol infection model. Although all strains grew exponentially in lungs, with similar bacterial burdens at the time of extrapulmonary dissemination, M. tuberculosis CDC1551 disseminated to the CNS significantly more than the H37Rv strains. No CNS lesions were observed throughout the study, with only a modest cytokine response. These data suggest that M. tuberculosis may have virulence factors that promote CNS dissemination, distinct from those required for pulmonary TB.

KW - Central nervous system

KW - Dissemination

KW - Guinea pig

KW - Meningitis

KW - Tuberculosis

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DO - 10.1016/j.tube.2011.07.003

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VL - 91

SP - 386

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JO - Tuberculosis

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ER -

Strain-dependent CNS dissemination in guinea pigs after Mycobacterium tuberculosis aerosol challenge

Abstract

Keywords

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