TY - JOUR
T1 - Stratification of clear cell renal cell carcinoma by signaling pathway analysis
AU - Cremona, Mattia
AU - Espina, Virginia
AU - Caccia, Dario
AU - Veneroni, Silvia
AU - Colecchia, Maurizio
AU - Pierobon, Mariaelena
AU - Deng, Jianghong
AU - Mueller, Claudius
AU - Procopio, Giuseppe
AU - Lanzi, Cinzia
AU - Daidone, Maria Grazia
AU - Cho, William C.S.
AU - Petricoin, Emanuel F.
AU - Liotta, Lance
AU - Bongarzone, Italia
N1 - Funding Information:
This work was supported by the Italian Istituto Superiore di Sanità in the framework Italy/USA cooperation agreement between the US Department of Health and Human Services, George Mason University, and the Italian Ministry of Public Health and by AIRC (Italian Association for Cancer Research).
PY - 2014/4
Y1 - 2014/4
N2 - Investigation of cell signaling pathways in 16 clear cell renal cell carcinomas to identify groups based on commonly shared phosphorylation-driven signaling networks. Using laser capture microdissection and reverse-phase protein arrays, we profiled 75 key nodes spanning signaling pathways important in tumorigenesis. Analysis revealed significantly different (P < 0.05) signaling levels for 27 nodes between two groups of samples, designated A (4 samples; high EGFR, RET, and RASGFR1 levels, converging to activate AKT/mTOR) and B (12 samples; high ERK1/2 and STAT phosphorylation). Group B was further partitioned into groups C (7 samples; elevated expression of LC3B) and D (5 samples; activation of Src and STAT). Network analysis indicated that group A was characterized by signaling pathways related to cell cycle and proliferation, and group B by pathways related to cell death and survival. Homogeneous clear cell renal cell carcinomas could be stratified into at least two major functional groups.
AB - Investigation of cell signaling pathways in 16 clear cell renal cell carcinomas to identify groups based on commonly shared phosphorylation-driven signaling networks. Using laser capture microdissection and reverse-phase protein arrays, we profiled 75 key nodes spanning signaling pathways important in tumorigenesis. Analysis revealed significantly different (P < 0.05) signaling levels for 27 nodes between two groups of samples, designated A (4 samples; high EGFR, RET, and RASGFR1 levels, converging to activate AKT/mTOR) and B (12 samples; high ERK1/2 and STAT phosphorylation). Group B was further partitioned into groups C (7 samples; elevated expression of LC3B) and D (5 samples; activation of Src and STAT). Network analysis indicated that group A was characterized by signaling pathways related to cell cycle and proliferation, and group B by pathways related to cell death and survival. Homogeneous clear cell renal cell carcinomas could be stratified into at least two major functional groups.
KW - HIF
KW - autophagy
KW - hypoxia
KW - mTOR
KW - renal cell carcinoma
KW - reverse-phase protein array
KW - signaling Putative biomarkers andpathway
UR - https://www.scopus.com/pages/publications/84896985961
U2 - 10.1586/14789450.2014.893193
DO - 10.1586/14789450.2014.893193
M3 - Article
C2 - 24575852
AN - SCOPUS:84896985961
SN - 1478-9450
VL - 11
SP - 237
EP - 249
JO - Expert Review of Proteomics
JF - Expert Review of Proteomics
IS - 2
ER -