@article{daa85a6e148a4c96bb97d196e448406a,
title = "Supramolecular organizations in the aerobic respiratory chain of Escherichia coli",
abstract = "The organization of respiratory chain complexes in supercomplexes has been shown in the mitochondria of several eukaryotes and in the cell membranes of some bacteria. These supercomplexes are suggested to be important for oxidative phosphorylation efficiency and to prevent the formation of reactive oxygen species. Here we describe, for the first time, the identification of supramolecular organizations in the aerobic respiratory chain of Escherichia coli, including a trimer of succinate dehydrogenase. Furthermore, two heterooligomerizations have been shown: one resulting from the association of the NADH:quinone oxidoreductases NDH-1 and NDH-2, and another composed by the cytochrome bo3 quinol:oxygen reductase, cytochrome bd quinol:oxygen reductase and formate dehydrogenase (fdo). These results are supported by blue native-electrophoresis, mass spectrometry and kinetic data of wild type and mutant E . coli strains.",
keywords = "Aerobic respiratory chain, Bioenergetics, Escherichia coli, NADH:quinone oxidoreductase, Oxygen reductase, Supercomplex",
author = "Sousa, {Pedro M.F.} and Silva, {Sara T.N.} and Hood, {Brian L.} and Nuno Charro and Carita, {Jo{\~a}o N.} and F{\'a}tima Vaz and Deborah Penque and Conrads, {Thomas P.} and Melo, {Ana M.P.}",
note = "Funding Information: We are greatly indebted to Drs. Miguel Teixeira and L{\'i}gia Saraiva for continuous suggestions during the work presented and critical review of this manuscript. The authors would like to thank Drs. Robert Gennis, Thorsten Friedrich and Joel Weiner for the E. coli strains ML20S2 and ML15A, ann001 and ann021, and DW35, respectively. Isabel Palos and Paula Alves, from IICT, and Patricia Gomes-Alves, from INSA are acknowledged for excellent technical assistance. The present research was funded by Funda {\c c}{\~a} o para a Ci {\^e} ncia e a Tecnologia (FCT) (PTDC/BIA-PRO/67105/2006 to AMPM), FCT/Poly-Annual Funding and FEDER/Sa {\'u} de XXI Programs to DP (Portugal); PS and NC received grants from FCT (SFRH/BD/46553/2008, SFRH/27906/2006, respectively), SS is the recipient of a BI grant in the frame of the financing project. ",
year = "2011",
month = mar,
doi = "10.1016/j.biochi.2010.10.014",
language = "English",
volume = "93",
pages = "418--425",
journal = "Biochimie",
issn = "0300-9084",
number = "3",
}