Targeted metagenomic assessment reflects critical colonization in battlefield injuries

Car Reen Kok; Nisha Mulakken; James B Thissen; Scott F Grey; Aram Avila-Herrera; Meenu M Upadhyay; Felipe A Lisboa; Shalini Mabery; Eric A Elster; Seth A Schobel; Nicholas A Be

doi:10.1128/spectrum.02520-23

Targeted metagenomic assessment reflects critical colonization in battlefield injuries

Car Reen Kok, Nisha Mulakken, James B Thissen, Scott F Grey, Aram Avila-Herrera, Meenu M Upadhyay, Felipe A Lisboa, Shalini Mabery, Eric A Elster, Seth A Schobel, Nicholas A Be

Research output: Contribution to journal › Article › peer-review

Abstract

Microbial contamination in combat wounds can lead to opportunistic infections and adverse outcomes. However, current microbiological detection has a limited ability to capture microbial functional genes. This work describes the application of targeted metagenomic sequencing to profile wound bioburden and capture relevant wound-associated signatures for clinical utility. Ultimately, the ability to detect such signatures will help guide clinical decisions regarding wound care and management and aid in the prediction of wound outcomes.

Original language	English
Pages (from-to)	e0252023
Journal	Microbiology Spectrum
Volume	11
Issue number	6
Early online date	24 Oct 2023
DOIs	https://doi.org/10.1128/spectrum.02520-23
State	Published - 12 Dec 2023

Keywords

Wound Infection/diagnosis
Humans
War-Related Injuries/diagnosis
Metagenome

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10.1128/spectrum.02520-23

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title = "Targeted metagenomic assessment reflects critical colonization in battlefield injuries",

abstract = "Microbial contamination in combat wounds can lead to opportunistic infections and adverse outcomes. However, current microbiological detection has a limited ability to capture microbial functional genes. This work describes the application of targeted metagenomic sequencing to profile wound bioburden and capture relevant wound-associated signatures for clinical utility. Ultimately, the ability to detect such signatures will help guide clinical decisions regarding wound care and management and aid in the prediction of wound outcomes.",

keywords = "Wound Infection/diagnosis, Humans, War-Related Injuries/diagnosis, Metagenome",

author = "Kok, {Car Reen} and Nisha Mulakken and Thissen, {James B} and Grey, {Scott F} and Aram Avila-Herrera and Upadhyay, {Meenu M} and Lisboa, {Felipe A} and Shalini Mabery and Elster, {Eric A} and Schobel, {Seth A} and Be, {Nicholas A}",

year = "2023",

month = dec,

day = "12",

doi = "10.1128/spectrum.02520-23",

language = "English",

volume = "11",

pages = "e0252023",

journal = "Microbiology Spectrum",

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T1 - Targeted metagenomic assessment reflects critical colonization in battlefield injuries

AU - Kok, Car Reen

AU - Mulakken, Nisha

AU - Thissen, James B

AU - Grey, Scott F

AU - Avila-Herrera, Aram

AU - Upadhyay, Meenu M

AU - Lisboa, Felipe A

AU - Mabery, Shalini

AU - Elster, Eric A

AU - Schobel, Seth A

AU - Be, Nicholas A

PY - 2023/12/12

Y1 - 2023/12/12

N2 - Microbial contamination in combat wounds can lead to opportunistic infections and adverse outcomes. However, current microbiological detection has a limited ability to capture microbial functional genes. This work describes the application of targeted metagenomic sequencing to profile wound bioburden and capture relevant wound-associated signatures for clinical utility. Ultimately, the ability to detect such signatures will help guide clinical decisions regarding wound care and management and aid in the prediction of wound outcomes.

AB - Microbial contamination in combat wounds can lead to opportunistic infections and adverse outcomes. However, current microbiological detection has a limited ability to capture microbial functional genes. This work describes the application of targeted metagenomic sequencing to profile wound bioburden and capture relevant wound-associated signatures for clinical utility. Ultimately, the ability to detect such signatures will help guide clinical decisions regarding wound care and management and aid in the prediction of wound outcomes.

KW - Wound Infection/diagnosis

KW - Humans

KW - War-Related Injuries/diagnosis

KW - Metagenome

U2 - 10.1128/spectrum.02520-23

DO - 10.1128/spectrum.02520-23

M3 - Article

C2 - 37874143

SN - 2165-0497

VL - 11

SP - e0252023

JO - Microbiology Spectrum

JF - Microbiology Spectrum

IS - 6

ER -

Targeted metagenomic assessment reflects critical colonization in battlefield injuries

Abstract

Keywords

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