Targeting Cre recombinase to specific neuron populations with bacterial artificial chromosome constructs

Shiaoching Gong; Martin Doughty; Carroll R. Harbaugh; Alexander Cummins; Mary E. Hatten; Nathaniel Heintz; Charles R. Gerfen

doi:10.1523/JNEUROSCI.2707-07.2007

Targeting Cre recombinase to specific neuron populations with bacterial artificial chromosome constructs

Shiaoching Gong
, Martin Doughty
, Carroll R. Harbaugh
, Alexander Cummins
, Mary E. Hatten
, Nathaniel Heintz
, Charles R. Gerfen^*

^*Corresponding author for this work

Anatomy, Physiology, and Genetics

Research output: Contribution to journal › Review article › peer-review

741 Scopus citations

Abstract

Transgenic mouse lines are characterized with Cre recombinase driven by promoters of CNS-specific genes using bacterial artificialchromosome (BAC) constructs. BAC-Cre constructs for 10 genes (Chat, Th, Slc6a4, Slc6a2, Etv1, Ntsr1, Drd2, Drd1, Pcp2, and Cmtm5)produced 14 lines with Cre expression in specific neuronal and glial populations in the brain. These Cre driver lines add functional utility to the >500 BAC-EGFP (enhanced green fluorescent protein) transgenic mouse lines that are part of the Gene Expression Nervous System Atlas Project.

Original language	English
Pages (from-to)	9817-9823
Number of pages	7
Journal	Journal of Neuroscience
Volume	27
Issue number	37
DOIs	https://doi.org/10.1523/JNEUROSCI.2707-07.2007
State	Published - 12 Sep 2007

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10.1523/JNEUROSCI.2707-07.2007

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title = "Targeting Cre recombinase to specific neuron populations with bacterial artificial chromosome constructs",

abstract = "Transgenic mouse lines are characterized with Cre recombinase driven by promoters of CNS-specific genes using bacterial artificialchromosome (BAC) constructs. BAC-Cre constructs for 10 genes (Chat, Th, Slc6a4, Slc6a2, Etv1, Ntsr1, Drd2, Drd1, Pcp2, and Cmtm5)produced 14 lines with Cre expression in specific neuronal and glial populations in the brain. These Cre driver lines add functional utility to the >500 BAC-EGFP (enhanced green fluorescent protein) transgenic mouse lines that are part of the Gene Expression Nervous System Atlas Project.",

author = "Shiaoching Gong and Martin Doughty and Harbaugh, \{Carroll R.\} and Alexander Cummins and Hatten, \{Mary E.\} and Nathaniel Heintz and Gerfen, \{Charles R.\}",

year = "2007",

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doi = "10.1523/JNEUROSCI.2707-07.2007",

language = "English",

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T1 - Targeting Cre recombinase to specific neuron populations with bacterial artificial chromosome constructs

AU - Gong, Shiaoching

AU - Doughty, Martin

AU - Harbaugh, Carroll R.

AU - Cummins, Alexander

AU - Hatten, Mary E.

AU - Heintz, Nathaniel

AU - Gerfen, Charles R.

PY - 2007/9/12

Y1 - 2007/9/12

N2 - Transgenic mouse lines are characterized with Cre recombinase driven by promoters of CNS-specific genes using bacterial artificialchromosome (BAC) constructs. BAC-Cre constructs for 10 genes (Chat, Th, Slc6a4, Slc6a2, Etv1, Ntsr1, Drd2, Drd1, Pcp2, and Cmtm5)produced 14 lines with Cre expression in specific neuronal and glial populations in the brain. These Cre driver lines add functional utility to the >500 BAC-EGFP (enhanced green fluorescent protein) transgenic mouse lines that are part of the Gene Expression Nervous System Atlas Project.

AB - Transgenic mouse lines are characterized with Cre recombinase driven by promoters of CNS-specific genes using bacterial artificialchromosome (BAC) constructs. BAC-Cre constructs for 10 genes (Chat, Th, Slc6a4, Slc6a2, Etv1, Ntsr1, Drd2, Drd1, Pcp2, and Cmtm5)produced 14 lines with Cre expression in specific neuronal and glial populations in the brain. These Cre driver lines add functional utility to the >500 BAC-EGFP (enhanced green fluorescent protein) transgenic mouse lines that are part of the Gene Expression Nervous System Atlas Project.

UR - https://www.scopus.com/pages/publications/34548651693

U2 - 10.1523/JNEUROSCI.2707-07.2007

DO - 10.1523/JNEUROSCI.2707-07.2007

M3 - Review article

C2 - 17855595

AN - SCOPUS:34548651693

SN - 0270-6474

VL - 27

SP - 9817

EP - 9823

JO - Journal of Neuroscience

JF - Journal of Neuroscience

IS - 37

ER -

Targeting Cre recombinase to specific neuron populations with bacterial artificial chromosome constructs

Abstract

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