@article{a643f3b5b27a4e538d130e21a7298941,
title = "Targeting the metastatic niche: Single-cell lineage tracing in prime time",
abstract = "Identification of actionable drug targets remains a rate-limiting step of, and one of the most prominent barriers to successful drug development for metastatic cancers. CRISPR-Cas9, a tool for making targeted genomic edits, has given rise to various novel applications that have greatly accelerated discovery in developmental biology. Recent work has coupled a CRISPR-Cas9-based lineage tracing platform with single-cell transcriptomics in the unexplored context of cancer metastasis. In this perspective, we briefly reflect on the development of these distinct technological advances and the process by which they have become integrated. We also highlight the importance of single-cell lineage tracing in oncology drug development and suggest the profound capacity of a high-resolution, computational approach to reshape cancer drug discovery by enabling identification of novel metastasis-specific drug targets and mechanisms of resistance.",
keywords = "Bioinformatics, Cancer, Genomic analysis, Phylogenetics",
author = "Sommer, {Elijah R.} and Napoli, {Giulia C.} and Chau, {Cindy H.} and Price, {Douglas K.} and Figg, {William D.}",
note = "Funding Information: This work was supported by the Intramural Research Program of the Center for Cancer Research, National Cancer Institute, National Institutes of Health (ZIA BC 010547). The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does it mention of trade names, commercial products, or organization imply endorsement by the U.S. Government. Conceptualization, E.R.S, C.H.C. W.D.F.; Writing—Original Draft, E.R.S, G.C.N. and C.H.C.; Writing—Review and Editing, E.R.S, G.C.N. C.H.C. D.K.P. and W.D.F.; Funding Acquisition, W.D.F.; Supervision, C.H.C. and W.D.F. All authors contributed to the article and approved the submitted version. None reported. Funding Information: This work was supported by the Intramural Research Program of the Center for Cancer Research , National Cancer Institute , National Institutes of Health ( ZIA BC 010547 ). The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does it mention of trade names, commercial products, or organization imply endorsement by the U.S. Government. Publisher Copyright: {\textcopyright} 2023",
year = "2023",
month = mar,
day = "17",
doi = "10.1016/j.isci.2023.106174",
language = "English",
volume = "26",
journal = "iScience",
issn = "2589-0042",
number = "3",
}